Retention of ingested porcine reproductive and respiratory syndrome virus in houseflies.

Objective: To evaluate retention of porcine reproductive and respiratory syndrome virus (PRRSV) in houseflies for various time frames and temperatures.

Sample Population: Fifteen 2-week-old pigs, two 10-week-old pigs, and laboratory-cultivated houseflies.

Procedure: In an initial experiment, houseflies were exposed to PRRSV; housed at 15 degrees, 20 degrees, 25 degrees, and 30 degrees C; and tested at various time points.

Spatial dispersal of porcine reproductive and respiratory syndrome virus-contaminated flies after contact with experimentally infected pigs.

Objective: To determine whether flies can acquire porcine reproductive and respiratory syndrome virus (PRRSV) and disperse the virus throughout a designated area.

Animals: 60 four-month-old pigs.

Procedure: On day 0, 28 of 60 pigs were inoculated with PRRSV MN 30-100 (index variant).