Transforming growth factor beta3 increases chondrocyte proliferation and decreases apoptosis in murine cricoid cartilage in vitro.

Objective: To determine if the luminal epithelium and/or exogenous transforming growth factor beta (TGFbeta) affects growth of the cricoid.

Design: Subglottises from 20 neonatal mice were subdivided into four groups: A, five subglottises with luminal epithelium grown in basic medium; B, five epithelium-free subglottises in basic medium; C, five epithelium-free subglottises in basic medium with supplemental TGFbeta1, and D, five epithelium-free subglottises in basic medium with supplemental TGFbeta3.

Results: Groups A and D demonstrated the greatest luminal area expansion.

Local control of murine subglottis development.

Objective: To determine if subglottic development is at least partially under local control and to determine which tissue layer(s) is predominantly responsible.

Design: The suglottises of 12 day-3 CD1 mice were grown in whole organ culture. The 12 subglottises were divided into 3 individual groups: +++, -++, and ---.