A Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of in the Field.

A real-time loop-mediated isothermal amplification (LAMP) assay for the detection of the causal pathogen of lettuce downy mildew, was developed and validated to aid in-field detection of airborne inoculum. Assay specificity was confirmed against a range of other pathogenic oomycete and fungal spp., and sensitivity of the assay for the detection of DNA extracted from sporangia was evaluated.