Matrix-assisted laser desorption mass spectrometry imaging for the examination of imipramine absorption by Straticell-RHE-EPI/001 an artificial model of the human epidermis.

In order to assess the potential of matrix-assisted laser desorption mass spectrometry imaging for the examination of artificial skin models, the absorption of the tricyclic antidepressant imipramine into Straticell-RHE-EPI/001 an artificial model of the human epidermis has been studied. The presence of imipramine could be clearly discerned in treated samples by imaging the distribution of the protonated molecule at m/z 281.18 in samples taken 2 and 8 h after treatment.

Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections.

During early-stage drug development, drug and metabolite distribution studies are carried out in animal tissues using a range of techniques, particularly whole body autoradiography (WBA). While widely employed, WBA has a number of limitations, including the following: expensive synthesis of radiolabeled drugs and analyte specificity and identification. WBA only images the radiolabel.