Variations in reactive oxygen release and antioxidant activity in multiple Symbiodinium types in response to elevated temperature.

As ocean temperatures rise, investigations into what the physiological effects will be on the symbiotic microalga Symbiodinium, and how these may play into the cnidarian bleaching response, have highlighted the contribution of reactive oxygen species (ROS). Previous studies have laid this groundwork using a limited number of Symbiodinium phylotypes, and so this study aims to expand this understanding by exploring the effects of sub-lethal elevated temperatures on the physiological response of seven genetically distinct types of Symbiodinium, including A1, B1, B2, C1, D, E1, and F2. The production of ROS (at 26 °C, 29 °C, 30 °C, and 31 °C) and activity of the antioxidants catalase (CAT) and superoxide dismutase (SOD) (at 26 °C and 31 °C) were measured as indicators of sensitivity or tolerance to heat stress.