Publications by authors named "Jenna Dennis"
Article Synopsis
- The study investigated the impact of cardiopulmonary resuscitation (CPR) on brain and skin oxygen levels in pigs experiencing severe hemorrhagic shock, a condition where blood pressure drops critically.
- Using a controlled experiment with 12 swine, results indicated that while CPR increased systolic blood pressure, it did not improve overall tissue oxygenation and, in fact, reduced skin perfusion significantly.
- The findings support previous research suggesting that CPR may not benefit organ oxygenation during hemorrhagic shock and could even have harmful effects.
Introduction: Adipose-derived stem cells (ASCs) are multipotent stem cells capable of differentiating into many cell lineages. They play an important role in wound healing by secreting cytokines. Prior studies have demonstrated the presence of proinflammatory cytokines in burn wounds.
View Article and Find Full Text PDFPyoderma gangrenosum (PG) is a rare, chronic, ulcerative disease characterized by non-healing wounds that worsen with debridement, a phenomenon called pathergy. No consensus regarding pathogenesis, diagnosis, or treatment exists for PG. A previous pilot study using dehydrated human amniotic/chorionic membrane (dHACM), following excisional debridement, augmented PG wound healing and allowed for subsequent wound closure through split-thickness skin grafting (STSG).
View Article and Find Full Text PDFBackground: Vascular endothelial growth factor is associated with reduced immune response and impaired anti-tumor activity. Combining antiangiogenic agents with immune checkpoint inhibition can overcome this immune suppression and enhance treatment efficacy.
Methods: This study investigated the combination of ziv-aflibercept anti-angiogenic therapy with pembrolizumab in patients with advanced melanoma resistant to anti-PD-1 treatment.
We developed a comprehensive method for functional assessment of the changes in immune populations and killing activity of peripheral blood mononuclear cells after cocultures with cancer cells using mass cytometry. In this study, a 43-marker mass cytometry panel was applied to a coculture of immune cells from healthy donors' peripheral blood mononuclear cells with diverse cancer cell lines. DNA content combined with classical CD45 surface staining was used as gating parameters for cocultures of immune cells (CD45/DNA) with hematological (CD45/DNA) and solid cancer cell lines (CD45/DNA).
View Article and Find Full Text PDFWith the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all mass cytometry expertise levels, aimed to generate reproducible datasets for large clinical cohorts. In this protocol, we provide details on the requirements to obtain meaningful results, spanning from sample quality, barcoding, and batch-freezing of stained samples.
View Article and Find Full Text PDFArticle Synopsis
- This protocol outlines a staining technique for analyzing immune cells in human bone marrow using mass cytometry.
- It addresses challenges like low cell viability and fragile cell samples to help obtain viable single cells for analysis.
- The assay is useful for studying immune cell characteristics, including activation and cytotoxicity, enhancing the understanding of human bone marrow in clinical contexts.