Cloning of the Bacillus subtilis lys and spoIIIB genes in phage phi 105.

The lys gene of Bacillus subtilis was inserted into prophage phi 105. The recombinant phage (phi 105dlys) contained DNA which was about 2 MDal smaller than the wild-type phage DNA, and the phage particles had no tails. The phage did not plaque but, when provided with tails in vitro, it transduced both lys-1 and lys-3 strains of B.

Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis.

Spores produced by a mutant of Bacillus subtilis were slow to develop their resistance properties during sporulation, and were slower to germinate than were wild-type spores. The coat protein composition of the mutant spores, as analysed by SDS-PAGE, was similar to that of the wild-type spores. However, one of the proteins (mol.

Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them.

During sporulation in replacement medium, resistance to toluene to heating at 65 degrees C, to lysozyme, and to heating at 80 degrees C appeared in sequence between 4 and 8 h after the induction of sporulation (i.e., between t4 and t8).

Interaction between drugs and potassium-rich solutions in producing contraction in bovine tracheal smooth muscle: studies in normal and calcium-depleted tissues.

1. The contracture normally induced in isolated bovine tracheal smooth muscle by potassium-rich solution was abolished by removal of the extracellular calcium. The contraction returned when calcium was added to the solution in a concentration greater than 0.