Publications by authors named "Jenika D Marshall"
Lipoprotein lipase (LPL) is upregulated in atherosclerotic lesions and it may promote the progression of atherosclerosis, but the mechanisms behind this process are not completely understood. We previously showed that the phosphorylation of Akt within THP-1 macrophages is increased in response to the lipid hydrolysis products generated by LPL from total lipoproteins. Notably, the free fatty acid (FFA) component was responsible for this effect.
View Article and Find Full Text PDFBackground: Formate can be incorporated into 10-formyl-tetrahydrofolate (10-formyl-THF), which is a substrate for purine synthesis, and after further reduction of the one-carbon group, may be used as a substrate for thymidylate synthesis and for homocysteine remethylation.
Objective: We examined plasma formate concentrations and the expression of genes involved in the production and utilization of formate in fetal and neonatal rats and in pregnant and virgin female rats.
Methods: In 1 experiment, plasma formate was measured by GC-MS in rats aged 1-56 d.
Article Synopsis
- - The study investigates whether a synthetic amino acid sequence similar to the heparin binding domain (HBD) of human hepatic lipase (hHL) can effectively displace hHL from cell surfaces.
- - Researchers created a recombinant protein with the specific HBD and found that it successfully removed hHL from HEK-293 cells, while a scrambled peptide did not have this effect.
- - While the HBD peptide was largely unstructured, its interaction with heparin showed potential changes in structure, indicating that mimetic peptides could be useful for targeting cell surface lipases.
Article Synopsis
- Macrophage lipoprotein lipase (LPL) contributes to fat buildup and atherosclerosis, but the specific effects of LPL's breakdown products on foam cell formation are unclear.
- A study using microarray analyses found that LPL hydrolysis products significantly altered the expression of many genes related to cell functions, stress responses, and lipid metabolism in human THP-1 macrophages.
- Results showed that the free fatty acids alone did not replicate the same gene regulation effects as the hydrolysis products, indicating that the impact on gene expression is dependent on the unique combinations of lipid species rather than just free fatty acids.