High-density cultivation of insect cells and production of recombinant baculovirus using a novel oscillating bioreactor.

A novel two-compartment bioreactor, BelloCell((R)), was used to cultivate insect cells and a maximum yield of 4.6 x 10(9) cells was attained. The cells were immobilized in a packed bed fixed in the upper chamber, and the bellow in the lower chamber was compressed and released in an alternating fashion.

A novel oscillating bioreactor BelloCell: implications for insect cell culture and recombinant protein production.

BelloCell is a novel packed bed bioreactor that allows alternating nutrient and gas transfer to a culture. Spodoptera frugiperda Sf-9 grown in the BelloCell (300 ml culture) reached 1.3-1.