Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer-Modified Magnetic Beads and Permalloy Micromagnets.

This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used with aptamer-modified magnetic beads (MBs) of various sizes to successfully separate lung cancer cells from a mixture of other cells. The overall separation efficiency was evaluated based on the ratios of cells in the different outlets and inlets of the chip.

Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer-based surface modification.

Metastasis remains a significant cause to cancer-related mortality, underscoring the critical need for early detection and analysis of circulating tumor cells (CTCs). This study presents a novel microfluidic chip designed to efficiently capture A549 lung cancer cells by combining dielectrophoresis (DEP) and aptamer-based binding, thereby enhancing capture efficiency and specificity. The microchip features interdigitated electrodes made of indium-tin-oxide that generate a nonuniform electric field to manipulate CTCs.

The effect of magnetic bead size on the isolation efficiency of lung cancer cells in a serpentine microchannel with added cavities.

An investigation was conducted to examine the effect of magnetic bead (MB) size on the effectiveness of isolating lung cancer cells using the immunomagnetic separation (IMS) method in a serpentine microchannel with added cavities (SMAC) structure. Carboxylated magnetic beads were specifically conjugated to target cells through a modification procedure using aptamer materials. Cells immobilized with different sizes (in micrometers) of MBs were captured and isolated in the proposed device for comparison and analysis.

Computational Intelligence in Cancer Diagnostics: A Contemporary Review of Smart Phone Apps, Current Problems, and Future Research Potentials.

Cancer is a dangerous and sometimes life-threatening disease that can have several negative consequences for the body, is a leading cause of mortality, and is becoming increasingly difficult to detect. Each form of cancer has its own set of traits, symptoms, and therapies, and early identification and management are important for a positive prognosis. Doctors utilize a variety of approaches to detect cancer, depending on the kind and location of the tumor.

A dielectrophoresis-based platform of cancerous cell capture using aptamer-functionalized gold nanoparticles in a microfluidic channel.

In this paper, a microfluidic chip for the manipulation and capture of cancer cells was introduced, in which the combination of dielectrophoresis (DEP) and a binding method based on chemical interactions by using cell-specific aptamers was performed to enhance the capture strength and specificity. The device has been simply constructed from a straight-channel PDMS placed on a glass substrate that has patterned electrode structures and a self-assembled monolayer of gold nanoparticles (AuNPs). The target cells were transported to the manipulation area by flow and attracted down to the region between the electrodes under the influence of positive DEP force.

Studies on Numerical Buckling Analysis of Cellulose Microfibrils Reinforced Polymer Composites.

Scientists are drawn to the new green composites because they may demonstrate qualities that are comparable to those of composites made of synthetic fibers due to concerns about environmental contamination. In this work, the potential for using the produced green composite in different buckling load-bearing structural applications is explored. The work on composite buckling characteristics is vital because one needs to know the composite's structural stability since buckling leads to structural instability.

Insights on Spark Plasma Sintering of Magnesium Composites: A Review.

This review paper gives an insight into the microstructural, mechanical, biological, and corrosion resistance of spark plasma sintered magnesium (Mg) composites. Mg has a mechanical property similar to natural human bones as well as biodegradable and biocompatible properties. Furthermore, Mg is considered a potential material for structural and biomedical applications.

Enhanced immunoassay in a nanofluidic preconcentrator utilizing nano-interstices among self-assembled gold nanoparticles.

In this study, we developed a microfluidic device for a dual work of protein preconcentration and subsequent capture by an immunoassay system. The fabrication of nano-interstices (nanochannels) to generate ion concentration polarization effect (ICP) for the preconcentration was simply performed by exploiting the loose association of glass-on-modified AuNPs to the polydimethylsiloxane (PDMS) channel pads as well as spaces between the substrate and the PDMS pad caused by these AuNPs. As a result, 65-fold concentration enhancement was achieved when performed on a sample of fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA).

Reviewing Performance Measures of the Die-Sinking Electrical Discharge Machining Process: Challenges and Future Scopes.

The most well-known and widely used non-traditional manufacturing method is electrical discharge machining (EDM). It is well-known for its ability to cut rigid materials and high-temperature alloys that are difficult to machine with traditional methods. The significant challenges encountered in EDM are high tool wear rate, low material removal rate, and high surface roughness caused by the continuous electric spark generated between the tool and the workpiece.

Powder Metallurgical Processing and Characterization of Molybdenum Addition to Tungsten Heavy Alloys by Spark Plasma Sintering.

The effect of adding molybdenum to the heavy tungsten alloy of W-Ni-Fe on its material characteristics was examined in the current study. The elemental powders of tungsten, iron, nickel, and molybdenum, with a composition analogous to W-3Fe-7Ni-xMo (x = 0, 22.5, 45, 67.

Immunomagnetic separation in a novel cavity-added serpentine microchannel structure for the selective isolation of lung adenocarcinoma cells.

The manipulation and separation of circulating tumor cells (CTCs) in continuous fluidic flows play an essential role in various biomedical applications, particularly the early diagnosis and treatment of diseases. Recent advances in magnetic bead development have provided promising solutions to the challenges encountered in CTC manipulation and isolation. In this study, we proposed a biomicrofluidic platform for specifically isolating human lung carcinoma A549 cells in microfluidic channels.

Effect of Wettability and Uniform Distribution of Reinforcement Particle on Mechanical Property (Tensile) in Aluminum Metal Matrix Composite-A Review.

There is a massive demand for low-weight high strength materials in automotive, space aerospace, and even structural industries in this present engineering world. These industries attract composites only because of their high strength, resistance to wear, and low weight. Among these composites, metal matrix composite finds wide applications due to its elevated properties, excellent resistance property, corrosion resistance, etc.

Effect of Molybdenum (Mo) Addition on Phase Composition, Microstructure, and Mechanical Properties of Pre-Alloyed Ti6Al4V Using Spark Plasma Sintering Technique.

The effect of molybdenum additions on the phases, microstructures, and mechanical properties of pre-alloyed Ti6Al4V was studied through the spark plasma sintering technique. Ti6Al4V-xMo (where x = 0, 2, 4, 6 wt.% of Mo) alloys were developed, and the sintered compacts were characterized in terms of their phase composition, microstructure, and mechanical properties.

Experimental and Numerical Analyses on the Buckling Characteristics of Woven Flax/Epoxy Laminated Composite Plate under Axial Compression.

The evolution of a sustainable green composite in various loadbearing structural applications tends to reduce pollution, which in turn enhances environmental sustainability. This work is an attempt to promote a sustainable green composite in buckling loadbearing structural applications. In order to use the green composite in various structural applications, the knowledge on its structural stability is a must.

Effect of Rare Earth Metals (Y, La) and Refractory Metals (Mo, Ta, Re) to Improve the Mechanical Properties of W-Ni-Fe Alloy-A Review.

Tungsten heavy alloys are two-phase metal matrix composites that include W-Ni-Fe and W-Ni-Cu. The significant feature of these alloys is their ability to acquire both strength and ductility. In order to improve the mechanical properties of the basic alloy and to limit or avoid the need for post-processing techniques, other elements are doped with the alloy and performance studies are carried out.

Effect of Nano Copper on the Densification of Spark Plasma Sintered W-Cu Composites.

In the present work, nano Cu (0, 5, 10, 15, 20, 25 wt.%) was added to W, and W-Cu composites were fabricated using the spark plasma sintering (S.P.

Effective Isolation for Lung Carcinoma Cells Based on Immunomagnetic Separation in a Microfluidic Channel.

In this paper, we developed an isolation system for A549 human lung carcinoma cells as an effective factor for the early diagnosis of lung cancer. A microfluidic immunomagnetic method was used, in which the combination of immunomagnetic separation and a microfluidic system allowed for increased isolation efficiency with uncomplicated manipulation. In the microfluidic immunomagnetic strategy, A549 cells were combined with aptamer-conjugated carboxylated magnetic beads and then collected in a specified region by applying a magnetic field.

Effect of Heating Modes on Reactive Sintering of CaCoO Ceramics.

The traditional solid-state reaction method was employed to synthesize bulk calcium cobaltite (Ca349/CaCoO) ceramics via ball milling the precursor mixture. The samples were compacted using conventional sintering (CS) and spark plasma sintering (SPS) at 850, 900, and 950 °C. The X-ray diffraction (XRD) pattern indicates the presence of the Ca349 phase for samples sintered at 850 and 900 °C.

Dynamic Characteristics of Woven Flax/Epoxy Laminated Composite Plate.

Due to the growing environmental awareness, the development of sustainable green composites is in high demand in composite industries, mainly in the automotive, aircraft, construction and marine applications. This work was an attempt to experimentally and numerically investigate the dynamic characteristics of Woven Flax/Bio epoxy laminated composite plates. In addition, the optimisation study on the dynamic behaviours of the Woven Flax/Bio epoxy composite plate is carried out using the response surface methodology (RSM) by consideration of the various parameters like ply orientation, boundary condition and aspect ratio.

Biological Living Cell in-Flow Detection Based on Microfluidic Chip and Compact Signal Processing Circuit.

Detection and counting of biological living cells in continuous fluidic flows play an essential role in many applications for early diagnosis and treatment of diseases. In this regard, this study highlighted the proposal of a biochip system for detecting and enumerating human lung carcinoma cell flow in the microfluidic channel. The principle of detection was based on the change of impedance between sensing electrodes integrated in the fluidic channel, due to the presence of a biological cell in the sensing region.

CuO/PEDOT:PSS/ZnO Nanocomposite Material Biosensor for Esophageal Cancer Detection.

A highly sensitive photoelectrochemical (PEC) biosensor without external bias was developed in this study. The biosensor was configured with a p-CuO and n-ZnO heterostructure. Hexamethylenetetramine (HMTA) and poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) was used to improve the crystal structure of CuO and ZnO and reduce the defects in the CuO/ZnO interface.

Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles.

This study established a microfluidic chip for the capture of A549 human lung circulating tumor cells via the aptamer-conjugated self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) in the channel. AuNPs are among the most attractive nanomaterials for the signal enhancement of biosensors owing to their unique chemical, physical, and mechanical properties. The microchip was fabricated using soft photolithography and casting and molding techniques.

Development of a Wireless Mesh Sensing System with High-Sensitivity LiNbO₃ Vibration Sensors for Robotic Arm Monitoring.

In recent years, multi-axis robots are indispensable in automated factories due to the rapid development of Industry 4.0. Many related processes were required to have the increasing demand for accuracy, reproducibility, and abnormal detection.

An Aptamer-Based Capacitive Sensing Platform for Specific Detection of Lung Carcinoma Cells in the Microfluidic Chip.

Improvement of methods for reliable and early diagnosis of the cellular diseases is necessary. A biological selectivity probe, such as an aptamer, is one of the candidate recognition layers that can be used to detect important biomolecules. Lung cancer is currently a typical cause of cancer-related deaths.

Early identification of esophageal squamous neoplasm by hyperspectral endoscopic imaging.

Esophageal squamous neoplasm presents a spectrum of different diatheses. A precise assessment for individualized treatment depends on the accuracy of the initial diagnosis. Detection relies on comprehensive and accurate white-light, iodine staining, and narrow-band imaging endoscopy.