Optimization of sugar release from sweet sorghum bagasse following solvation of cellulose and enzymatic hydrolysis using response surface methodology.

To release sugars effectively from sweet sorghum bagasse (SSB), a cellulose solvent and organic solvent-based lignocellulose fractionation pretreatment approach was studied using response surface methodology (RSM). Based on RSM's central composite design, a batch experimental matrix was set up to determine the effects of reaction time (20-60 min) and temperature (40-60 °C) on delignification, total reducing sugar yield, glucan digestibility, and overall glucose yields following a pretreatment-hydrolysis process. The optimum pretreatment conditions of 50 °C and 40 min led to 51.

Fermentable sugar release from Jatropha seed cakes following lime pretreatment and enzymatic hydrolysis.

Composition change of Jatropha seed cake samples was evaluated upon lime pretreatment at 100 degrees C with different parameters. With a lime dose of 0.2 g and a water content of 10 ml per gram of cake and a treatment period of 1 h, 38.

Use of sweet sorghum juice for lipid production by Schizochytrium limacinum SR21.

Stalk juice from sweet sorghum grown in Southern Illinois, USA, was examined for lipid production through microalgal fermentation. Juice concentrations at 100%, 75%, 50%, and 25% led to different biomass, lipid, and docosahexaenoic acid (DHA) production by Schizochytrium limacinum SR21. Biomass dry weight as 9.

Toward plant cell wall degradation under thermophilic condition: a unique microbial community developed originally from swine waste.

A unique thermophilic microbial community developed initially from swine waste was investigated in this study. Cellulase activities were observed when this community was inoculated to media containing either cellulose or carboxymethylcellulose at 57 degrees C. Through constructing a clone library for the 16S ribosomal DNA, it was revealed that this community was mainly composed of three genera: Thermobacillus, Brevibacillus, and Anoxybacillus.

Optimization of growth medium and enzyme assay conditions for crude cellulases produced by a novel thermophilic and cellulolytic bacterium, Anoxybacillus sp. 527.

A newly isolated Anoxybacillus sp. 527 was found to grow on crystalline cellulose as sole carbon and energy sources. Cellulases secreted by strain 527 were better induced by cellobiose, followed by glucose, lactose, sucrose, and cellulose.

Study of cellulases from a newly isolated thermophilic and cellulolytic Brevibacillus sp. strain JXL.

A potentially novel aerobic, thermophilic, and cellulolytic bacterium designated as Brevibacillus sp. strain JXL was isolated from swine waste. Strain JXL can utilize a broad range of carbohydrates including: cellulose, carboxymethylcellulose (CMC), xylan, cellobiose, glucose, and xylose.