Publications by authors named "Jelena D Pajovic"
Article Synopsis
- Gold nanoparticles were enhanced using the amino acid tryptophan and vitamin riboflavin, resulting in a 65% increase in resonance energy transfer (RET) efficiency.
- The presence of these nanoparticles altered the photobleaching dynamics of fluorescent molecules, making them detectable in biological materials with autofluorescent compounds.
- Using advanced deep-ultraviolet fluorescence microscopy, researchers could classify fluorescent centers in cancer cells to identify areas where the nanoparticles accumulated, despite their small size.
The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores.
View Article and Find Full Text PDFTryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells.
Colloids Surf B Biointerfaces
November 2015
Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid.
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