Resistome and virulome determination in using next-generation sequencing with target-enrichment technology.

The identification of infection from gastric biopsy samples requires PCR or bacterial cultures. However, it is difficult to culture because it is a fragile bacterium. Next-generation sequencing (NGS) allows direct assessment of the resistome and virulome.

The first two human infections with Helicobacter zhangjianzhongii, a new Helicobacter closely related to Helicobacter canis.

Purpose: In 2023, Helicobacter zhangjianzhongii was proposed as a new species in the Helicobacter genus. We here describe two human cases of H. zhangjianzhongii bacteremia.

Description of a Case of Infection with Resistance to Tetracycline: An Exceptional Event with No Consequences?

Resistance in to tetracycline is rare. We describe the case of an strain with a high level of resistance to tetracycline (minimum inhibitory concentration = 12 mg/L). However, despite tetracycline resistance, bismuth quadritherapy was effective.

Combined genomic-proteomic approach in the identification of amoxicillin-clavulanic acid resistance mechanism in clinical isolates.

Introduction: Aminopenicillins resistance among and strains is associated with a single mutation in the promoting region of a chromosomal beta-lactamase , allowing its expression. Clavulanic acid is used to restore aminopenicillins activity in case of expression and has also an inherent antimicrobial activity over spp. Resistance to amoxicillin-clavulanic acid is therefore extremely rare among these species: only 0.

foodborne illness outbreak in the elderly.

In June 2021, a cluster of seven cases of infections occurred in a rehabilitation center and caused significant morbidity in elderly patients including five with bacteremia and two with osteoarticular medical device infections. The genetic identity identified by whole genome sequencing of the different strains confirms a common source. This foodborne illness outbreak may have resulted from the consumption of unpasteurized dairy products, such as a cow's raw milk cheese resulting from a farm-to-fork strategy.

Recurrent Campylobacter jejuni Infections with Selection of Resistance to Macrolides and Carbapenems: Molecular Characterization of Resistance Determinants.

We present two independent cases of recurrent multidrug-resistant Campylobacter jejuni infection in immunocompromised hosts and the clinical challenges encountered due to the development of high-level carbapenem resistance. The mechanisms associated with this unusual resistance for were characterized. Initial macrolide and carbapenem-susceptible strains acquired resistance to erythromycin (MIC > 256mg/L), ertapenem (MIC > 32mg/L), and meropenem (MIC > 32mg/L) during treatment.

Molecular Cut-off Values for Aliarcobacter butzleri Susceptibility Testing.

Aliarcobacter butzleri is an emerging gastrointestinal pathogen found in many countries worldwide. In France, it has become the third most commonly isolated bacterial species from the stools of patients with intestinal infections. No interpretative criteria for antimicrobial susceptibility testing have been proposed for A.

Emergence of Erythromycin Resistance Methyltransferases in Campylobacter coli Strains in France.

Antimicrobial resistance in campylobacters has been described worldwide. The emergence of multiresistant isolates, particularly among Campylobacter coli isolates, is concerning. New resistance mechanisms appear frequently, and DNA-sequence-based methods such as whole-genome sequencing (WGS) have become useful tools to monitor their emergence.

Genome-Wide Identification of Host-Segregating Single-Nucleotide Polymorphisms for Source Attribution of Clinical Campylobacter coli Isolates.

is among the most common causes of gastroenteritis worldwide. and are the most common species causing human disease. DNA sequence-based methods for strain characterization have focused largely on , responsible for 80 to 90% of infections, meaning that epidemiology has lagged behind.

Evaluation of the Allplex™ H pylori and ClariR PCR Assay for Helicobacter pylori detection on gastric biopsies.

Background: The diagnosis of Helicobacter pylori infection can be made by PCR on gastric biopsies. The objective of this study was to evaluate retrospectively the performance of the Allplex™ H pylori and ClariR PCR Assay (Seegene).

Material And Methods: A collection of 180 DNA samples extracted from gastric biopsies was used in this study: 90 DNAs from H pylori-negative patients and 90 from H pylori-positive patients.

Rapid Characterization of Virulence Determinants in Isolated from Non-Atrophic Gastritis Patients by Next-Generation Sequencing.

is a major human pathogen that causes a wide range of gastrointestinal pathology. Progression of induced gastritis to more severe disease has been found to highly correlate with the array of virulence factors expressed by the pathogen. The objective of this study was twofold: first, to characterize the genetic diversity of strains isolated from 41 non-atrophic gastritis patients in Switzerland, an issue that has not been investigated to date.