Traditional methods to assess microbial cells during suspension culture require laborious and frequent manual sampling. Approaches to automate sampling and assessment utilize dedicated, sophisticated equipment and suffer from a lack of temporal resolution and sampling efficiency. In this study we describe a simple microfluidic device that allows microbial cells to be sampled from suspension culture and rapidly slowed and concentrated for single-cell imaging on a standard laboratory microscope.
View Article and Find Full Text PDFRobotic totally endoscopic coronary artery bypass grafting (TECAB) was introduced in 1998 and has over a period of two decades gradually emerged from single-vessel revascularization to multivessel bypass grafting. Dedicated centers have continuously evolved and further developed this minimally invasive method of coronary bypass surgery. A literature review was conducted to assess intra- and postoperative outcomes of TECAB.
View Article and Find Full Text PDFAnn Cardiothorac Surg
November 2016
Background: Robotically-assisted coronary bypass grafting (CABG) was introduced in 1998 and dedicated centers have continuously applied and developed this minimally invasive method of coronary bypass surgery. While short-term results are relatively well published, data on long-term outcome are limited. In this literature review, we assessed the outcomes after robotic CABG following the first postoperative year.
View Article and Find Full Text PDFAnn Otol Rhinol Laryngol
December 2016
Objectives: The objective of this study was to create a 3D printed airway model simulating the size and mechanical properties of various age groups for foreign body removal training.
Methods: Three-dimensional printing technology was used to print the anatomically correct airway from rubber-like translucent material, simulating the mechanical properties of human airway tissue. The model's effectiveness in trainee education was evaluated by otolaryngology residents with varying levels of experience.
Objective/hypothesis: The objective of this study was to determine the validity of our laryngopharyngeal reflux (LPR) diagnostic system from our previous study (Witt et al.) against the results of a standard pH probe monitoring. We hypothesized that subjects with abnormal pH probe results demonstrate color and texture abnormalities that would be classified as LPR according to artificial neural network (ANN) analysis.
View Article and Find Full Text PDFObjectives/hypothesis: Traditional semi-occluded vocal tract therapies have the benefit of improving vocal economy but, do not allow for connected speech during rehabilitation. In this study, we introduce a semi-occluded face mask (SOFM) as an improvement upon current methods. This novel technique allows for normal speech production, and will make the transition to everyday speech more natural.
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