Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers.

A recombinant 130 kDa dihemoglobin which is made up of a single-chain tetra-α globin and four β globins has been expressed as a soluble protein in E. coli. The sequence of the single chain tetra-α is: αI-Gly-αII-(SerGlyGly)5Ser-αIII-Gly-αIV.

In vitro elution studies of amikacin and cefazolin from polymethylmethacrylate.

Objective: To compare the in vitro elution characteristics of amikacin and cefazolin from polymethylmethacrylate (PMMA) alone and in combination.

Study Design: A prospective, controlled, experimental study.

Methods: Three aliquots of 6 g sterile PMMA were measured and to them added (1) 750 mg amikacin; (2) 1050 mg cefazolin; and (3) 750 mg amikacin and 1050 mg cefazolin.

Urodynamic effects of a percutaneously controlled static hydraulic urethral sphincter in canine cadavers.

Objective: To describe a percutaneously controlled static hydraulic urethral sphincter (SHUS) and evaluate urodynamic effects of the SHUS in canine cadavers.

Sample Population: Cadavers of 6 adult female dogs.

Procedure: Cadavers were obtained immediately after dogs were euthanatized.