Pulmonary matrix-derived hydrogels from patients with idiopathic pulmonary fibrosis induce a proinflammatory state in lung fibroblasts in vitro.

Idiopathic pulmonary fibrosis (IPF), one of the most common forms of interstitial lung disease, is a poorly understood, chronic, and often fatal fibroproliferative condition with only two FDA-approved medications. Understanding the pathobiology of the fibroblast in IPF is critical to evaluating and discovering novel therapeutics. Using a decellularized lung matrix derived from patients with IPF, we generate three-dimensional hydrogels as in vitro models of lung physiology and characterize the phenotype of fibroblasts seeded into the hydrogels.

Eliminating waste with waste: transforming spent coffee grounds into microrobots for water treatment.

Water pollutants such as oil spills, industrial dyes, and microplastics threaten public health and aquatic ecosystems. There are considerable challenges in removing water contaminants using traditional methods. Several studies have been conducted in recent years to develop effective water purification materials.

Active Synthetic Microrotors: Design Strategies and Applications.

Microrotors are microscopic objects that convert energy stored in the environment into spontaneous rotation, in the form of spinning along an axis, rolling on a surface, or orbiting in circles. Because of its distinct dynamics and the vertical flows around it, a microrotor is potentially useful for applications, including drug delivery, minimally invasive surgery, fluid mixing, and sensing. It is also useful as a model system to probe the collective behaviors among rotating micro-objects.

Development of a clinical and translational research curriculum for undergraduate students.

Introduction: Research participation during undergraduate years has a powerful influence on career selection and attitudes toward scientific research. Most undergraduate research programs in academic health centers are oriented toward basic research or address a particular disease focus or research discipline. Undergraduate research programs that expose students to clinical and translational research may alter student perceptions about research and influence career selection.

Size-tunable ICG-based contrast agent platform for targeted near-infrared photoacoustic imaging.

Near-infrared photoacoustic imaging (NIR-PAI) combines the advantages of optical and ultrasound imaging to provide anatomical and functional information of tissues with high resolution. Although NIR-PAI is promising, its widespread use is hindered by the limited availability of NIR contrast agents. J-aggregates (JA) made of indocyanine green dye (ICG) represents an attractive class of biocompatible contrast agents for PAI.

Generic Rules for Distinguishing Autophoretic Colloidal Motors.

Distinguishing the operating mechanisms of nano- and micromotors powered by chemical gradients, i.e. "autophoresis", holds the key for fundamental and applied reasons.

Autonomously Propelled Colloids for Penetration and Payload Delivery in Complex Extracellular Matrices.

For effective treatment of diseases such as cancer or fibrosis, it is essential to deliver therapeutic agents such as drugs to the diseased tissue, but these diseased sites are surrounded by a dense network of fibers, cells, and proteins known as the extracellular matrix (ECM). The ECM forms a barrier between the diseased cells and blood circulation, the main route of administration of most drug delivery nanoparticles. Hence, a stiff ECM impedes drug delivery by limiting the transport of drugs to the diseased tissue.

Chemokinesis-driven accumulation of active colloids in low-mobility regions of fuel gradients.

Many motile cells exhibit migratory behaviors, such as chemotaxis (motion up or down a chemical gradient) or chemokinesis (dependence of speed on chemical concentration), which enable them to carry out vital functions including immune response, egg fertilization, and predator evasion. These have inspired researchers to develop self-propelled colloidal analogues to biological microswimmers, known as active colloids, that perform similar feats. Here, we study the behavior of half-platinum half-gold (Pt/Au) self-propelled rods in antiparallel gradients of hydrogen peroxide fuel and salt, which tend to increase and decrease the rods' speed, respectively.

A practical guide to active colloids: choosing synthetic model systems for soft matter physics research.

Synthetic active colloids that harvest energy stored in the environment and swim autonomously are a popular model system for active matter. This emerging field of research sits at the intersection of materials chemistry, soft matter physics, and engineering, and thus cross-talk among researchers from different backgrounds becomes critical yet difficult. To facilitate this interdisciplinary communication, and to help soft matter physicists with choosing the best model system for their research, we here present a tutorial review article that describes, in appropriate detail, six experimental systems of active colloids commonly found in the physics literature.

Noble-gas-infused neoprene closed-cell foams achieving ultra-low thermal conductivity fabrics.

Closed-cell foams are widely applied as insulation and essential for the thermal management of protective garments for extreme environments. In this work, we develop and demonstrate a strategy for drastically reducing the thermal conductivity of a flexible, closed-cell polychloroprene foam to 0.031 ± 0.

Numerical study of the effect of soft layer properties on bacterial electroporation.

We present a numerical model of electroporation in a gram-positive bacterium, which accounts for the presence of a negatively charged soft polyelectrolyte layer (which may include a periplasmic space, peptidoglycan layer, cilia, flagella, and other surface appendages) surrounding its plasma membrane. We model the ion transport within and outside the soft layer using the soft layer electrokinetics-based Poisson-Nernst-Planck formalism. Additionally, we model the electroporation dynamics on the plasma membrane using the pore nucleation-based electroporation formalism developed by Krassowska and Filev.

Microfluidic Screening of Electric Fields for Electroporation.

Electroporation is commonly used to deliver molecules such as drugs, proteins, and/or DNA into cells, but the mechanism remains poorly understood. In this work a rapid microfluidic assay was developed to determine the critical electric field threshold required for inducing bacterial electroporation. The microfluidic device was designed to have a bilaterally converging channel to amplify the electric field to magnitudes sufficient to induce electroporation.

16th Annual Land O'Lakes Bioanalytical Conference.

This Land O'Lakes Conference is presented each year by the Division of Pharmacy Professional Development within the School of Pharmacy at the University of Wisconsin-Madison (USA). The purpose of this 3-day conference is to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference is designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis.

Summary of the 2014 Land O'Lakes Bioanalytical Conference.

This Land O'Lakes Conference is presented each year by the Division of Pharmacy Professional Development within the School of Pharmacy at the University of Wisconsin-Madison (USA). The purpose of this 3-day conference is to provide an educational forum to discuss issues and applications associated with the analysis of xenobiotics, metabolites, biologics and biomarkers in biological matrices. The conference is designed to include and encourage an open exchange of scientific and methodological applications for bioanalysis.

Rapid fabrication of bimetallic spherical motors.

Catalytic bimetallic nanomotors can swim at 100 body lengths per second as well as pick up, haul, and release micrometer-scale cargo. The electrokinetic locomotion of bimetallic nanomotors is driven by the electrocatalytic decomposition of hydrogen peroxide. The motors are typically fabricated by electrodeposition-based template synthesis techniques that result in heterogeneous samples and require specialized knowledge of electrochemistry, a three-electrode potentiostat setup, cyanide-based chemistry, and porous membranes.

Submicron scale patterning in sintered silica colloidal crystal films using a focused ion beam.

Focused ion beam milling is used to fabricate micron and submicron scale patterns in sintered silica colloidal crystal films. Rectangular cavities with both solid and porous boundaries, fluidic channels, and isolation of a small number of packed spheres are patterned. The ion beam can pattern sintered films of individual submicron size spheres and create patterns that cover up to 40 mum in less than 15 min.

Safety, pharmacokinetics, pharmacodynamics, and plasma lipoprotein distribution of eritoran (E5564) during continuous intravenous infusion into healthy volunteers.

Eritoran, a structural analogue of the lipid A portion of lipopolysaccharide (LPS), is an antagonist of LPS in animal and human endotoxemia models. Previous studies have shown that low doses (350 to 3,500 microg) of eritoran have demonstrated a long pharmacokinetic half-life but a short pharmacodynamic half-life. The present study describes the safety, pharmacokinetics and pharmacodynamics, and lipid distribution profile of eritoran during and after a 72-h intravenous infusion of 500, 2,000, or 3,500 microg/h into healthy volunteers.