Leukocyte telomere length and carotid artery intimal medial thickness: the Framingham Heart Study.

Background And Purpose: Leukocyte telomere length (LTL) is relatively short in individuals who have evidence of cardiovascular disease. The purpose of this study was to examine the link between LTL and the predisposition to atherosclerosis, as determined by carotid artery intimal medial thickness (IMT) in participants of the Framingham Offspring Study.

Methods And Results: LTL was assayed by the mean length of the terminal restriction fragments and carotid artery IMT by B-mode ultrasonography in 1062 individuals (496 men, 566 women) aged 33 to 86 years.

Lack of association between leukocyte telomere length and genetic variants in two ageing-related candidate genes.

Background: Leukocyte telomere length, a putative marker of ageing, is a highly variable and heritable complex trait. In order to determine the possible underlying genetic variants for leukocyte telomere length variation, we conducted an association study of leukocyte telomere length and two candidate genes for ageing-related traits, TGFB1 and KLOTHO, in a female Caucasian dizygotic twin population.

Methods And Materials: Terminal restriction fragment (TRF) length, an index of telomere length, was measured using Southern Blotting.