Development of a β-Lactoglobulin Sensor Based on SPR for Milk Allergens Detection.

A sensitive and label-free surface plasmon resonance (SPR) based sensor was developed in this work for the detection of milk allergens. β-lactoglobulin (BLG) protein was used as the biomarker for cow milk detection. This is to be used directly in final rinse samples of cleaning in-place (CIP) systems of food manufacturers.

Synthesis of Molecularly Imprinted Polymer Nanoparticles for α-Casein Detection Using Surface Plasmon Resonance as a Milk Allergen Sensor.

Food recalls due to undeclared allergens or contamination are costly to the food manufacturing industry worldwide. As the industry strives for better manufacturing efficiencies over a diverse range of food products, there is a need for the development of new analytical techniques to improve monitoring of the presence of unintended food allergens during the food manufacturing process. In particular, the monitoring of wash samples from cleaning in place systems (CIP), used in the cleaning of food processing equipment, would allow for the effective removal of allergen containing ingredients in between food batches.

Antiapoptotic serine protease inhibitors contribute to survival of allergenic T2 cells.

Background: The mechanisms that regulate maintenance of persistent T2 cells and potentiate allergic inflammation are not well understood.

Objective: The function of serine protease inhibitor 2A (Spi2A) was studied in mouse T2 cells, and the serine protease inhibitor B3 (SERPINB3) and SERPINB4 genes were studied in T2 cells from patients with grass pollen allergy.

Methods: Spi2A-deficient T2 cells were studied in in vitro culture or in vivo after challenge of Spi2A knockout mice with ovalbumin in alum.

Serine protease inhibitor 6 protects iNKT cells from self-inflicted damage.

The role played by apoptosis in the homeostasis of effector cells of the innate immune system is unclear. Serine protease inhibitor 6 (Spi6) is an inhibitor of granzyme B (GrB) that protects cytotoxic T lymphocytes of the adaptive immune system from apoptosis. To determine whether Spi6 also protects cells of the innate immune system from self-inflicted damage we have examined invariant NKT (iNKT) cells.