Complete genome sequence and the expression pattern of plasmids of the model ethanologen ZM4 and its xylose-utilizing derivatives 8b and 2032.

Background: is a natural ethanologen being developed and deployed as an industrial biofuel producer. To date, eight strains have been completely sequenced and found to contain 2-8 native plasmids. However, systematic verification of predicted plasmid genes and their contribution to cell fitness has not been hitherto addressed.

Mapping E. coli RNA polymerase and associated transcription factors and identifying promoters genome-wide.

The ability to examine gene regulation in living cells has been greatly enabled by the development of chromatin immunoprecipitation (ChIP) methodology. ChIP captures a snapshot of protein-DNA interactions in vivo and has been used to study interactions in bacteria, yeast, and mammalian cell culture. ChIP conditions vary depending upon the organism and the nature of the DNA-binding proteins under study.

GATA2 functions at multiple steps in hemangioblast development and differentiation.

Molecular mechanisms that regulate the generation of hematopoietic and endothelial cells from mesoderm are poorly understood. To define the underlying mechanisms, we compared gene expression profiles between embryonic stem (ES) cell-derived hemangioblasts (Blast-Colony-Forming Cells, BL-CFCs) and their differentiated progeny, Blast cells. Bioinformatic analysis indicated that BL-CFCs resembled other stem cell populations.