Opiate screening and quantitation in urine/blood matrices using LC-MS/MS techniques.

Here we describe a high-volume urinary screening technique for opiate drugs as well as other narcotic analgesics. We also describe methods for quantification of the same drug species in serum, plasma, and whole blood. Screening and quantitation of these types of drugs have presented many challenges, among them the potentially low levels in both abuse and therapeutic situations.

LC-MS/MS techniques for high-volume screening of drugs of abuse and target drug quantitation in urine/blood matrices.

Liquid chromatography-tandem mass spectrometry, employing electrospray ionization (ESI), has been applied in the analysis of many drugs and drug metabolites. Sample preparation has been an important part of this technique when analyzing biological samples. Here we describe a high-volume urine screening technique for approximately 40 different drugs of abuse as well as methods for quantification of many other drugs in serum, plasma, and whole blood.

Drugs of abuse testing by tandem mass spectrometry: a rapid, simple method to replace immunoassays.

Primary Objective: To replace immunoassay screening for drugs of abuse (DOA) with a cost-effective tandem mass spectrometry method.

Secondary Objective: To substantially expand the drugs of abuse assay menu.

Design And Methods: The requirement was to perform high throughput DOA screening for 200 urine specimens/day for 40 drugs/metabolites.

Formate pharmacokinetics during formate administration in folate-deficient young swine.

The objective of the study was to investigate the effect of folate deficiency on formate pharmacokinetics during formate administration in folate-deficient young swine. Methanol is a one of the congeners found in alcoholic beverages. Methanol toxicity is mediated through formic acid and thus plays a significant role in the pathophysiology of alcoholism.