A detailed comparative in silico and functional analysis of ccd1 gene in maize gives new insights of its expression and functions.

Background: Biofortified maize with enhanced carotenoid content was developed to combat vitamin A deficiency. However, it was observed that during storage, carotenoids present in maize grain get degraded and it has been reported that carotenoid cleavage dioxygenase1 (ccd1) is responsible for this degradation.

Methods And Results: In our current study, comprehensive in-silico analysis deciphered a complete overview of the ccd1 gene in maize including the gene structures, phylogeny, chromosomal locations, promoter analysis, conserved motifs and interacting protein partners.

Low titre of agroinoculum with prolonged incubation period and low auxin concentration in the regeneration media are the key to high frequency of transformation in climate-resilient -type rice genotype Nagina 22.

Unlabelled: Developing an efficient and reproducible regeneration protocol holds paramount significance for advancing genetic transformation technologies in rice, facilitating their utilisation in crop improvement. Nagina 22 (N22), a climate-resilient -type rice genotype known for its tolerance against multiple stresses, lacks a standardised transformation protocol, limiting its utilisation as a background for genetic transformation. This study reports, for the first time, a highly efficient transformation and regeneration protocol for N22 using a CRISPR/Cas9 vector.