Publications by authors named "Jeanne-Marie Libouton"
Patterns of genomic aberrations suggest that Burkitt lymphomas with complex karyotype are distinct from other aggressive B-cell lymphomas with MYC rearrangement.
Genes Chromosomes Cancer
January 2013
Article Synopsis
- The study investigates the negative impact of complex karyotypes and chromosome 13q abnormalities on prognosis in childhood Burkitt lymphomas (BL) and diffuse large B-cell lymphomas (DLBCL).
- Using multicolor fluorescence in situ hybridization (M-FISH), researchers analyzed 84 patient samples and discovered new chromosomal abnormalities in 80% of them, enhancing understanding of genetic alterations linked to MYC rearrangements.
- The results highlight distinct patterns of genomic changes across different lymphoma types, indicating that B-cell lymphoma subtypes may have varying degrees of complexity and prognosis, particularly identifying DLBCL/BL as a unique category.
Fluorescence in situ hybridization was performed to characterize 81 cases of myeloid and lymphoid malignancies with cytogenetic 1p36 alterations not affecting the PRDM16 locus. In total, three subgroups were identified: balanced translocations (N = 27) and telomeric rearrangements (N = 15), both mainly observed in myeloid disorders; and unbalanced non-telomeric rearrangements (N = 39), mainly observed in lymphoid proliferations and frequently associated with a highly complex karyotype. The 1p36 rearrangement was isolated in 12 cases, mainly myeloid disorders.
View Article and Find Full Text PDFWe performed a multicentric study to assess the impact of two different culture procedures on the detection of chromosomal abnormalities in 217 consecutive unselected cases with chronic lymphocytic leukemia (CLL) referred for routine analysis either at the time of diagnosis (n = 172) or during disease evolution (n = 45). Parallel cultures of peripheral blood or bone marrow were set up with the addition of either the conventional B-cell mitogen 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or a combination of CpG oligonucleotide (CpG) and interleukin-2 (IL-2). Cytogenetic analyses were performed on both cultures.
View Article and Find Full Text PDFPurpose: The present study aimed at investigating if 2'-2' difluorodeoxycytidine (dFdC) radioenhancement was mediated by an effect on induction and/or repair of radiation-induced DNA DSBs and chromosome aberrations in cells with different intrinsic radiosensitivity.
Methods: Confluent human head and neck squamous cell carcinoma cell lines designated SCC61 and SQD9 were treated with 5 microM dFdC for 3 or 24 h prior to irradiation. DNA DSBs induction and repair were analyzed by PFGE.