Evaluation of scale-up effect on cold-active enzyme production and biodegradation tests using pilot-scale bioreactors and a 3D soil tank.

Despite recent attention being paid to the biodegradation of petroleum hydrocarbons in cold environments, scale-up studies of biodegradation are lacking. Herein, the effect of scale-up on the enzymatic biodegradation of highly contaminated soil at low temperatures was studied. A novel cold-adapted bacteria (Arthrobacter sp.

Pilot-scale production and in-situ application of petroleum-degrading enzyme cocktail from Alcanivorax borkumensis.

Petroleum degrading enzymes can be used as an alternative way to improve petroleum bioremediation approaches. Alcanivorax borkumensis is an alkane-degrading bacteria that can produce petroleum degrading enzymes such as alkane hydroxylase and lipase. In this study, pilot-scale Alcanivorax borkumensis fermentation was developed for producing large volumes of petroleum degrading enzymes cocktail (∼900 L).

Column tests for evaluation of the enzymatic biodegradation capacity of hydrocarbons (C-C) contaminated soil.

Though many studies pertaining to soil bioremediation have been performed to study the microbial kinetics in shake flasks, the process efficiency in column tests is seldom. In the present study, soil columns tests were carried out to study the biodegradation of soil contaminated with a high concentration of diesel (≈19.5 g/kg) petroleum hydrocarbons expressed as C-C.

Bench-scale production of enzymes from the hydrocarbonoclastic bacteria Alcanivorax borkumensis and biodegradation tests.

This study investigates motor oil (3, 5, 7.5 and 10% (v v)) as a sole carbon source for the production of Alcanivorax borkumensis in shake flasks and a 5 L bench-scale fermenter in comparison to the standard media. Shake flask studies showed a significant and higher cell growth (p=0.

Production and characterization of novel hydrocarbon degrading enzymes from Alcanivorax borkumensis.

This study investigates the production of alkane hydroxylase, lipase and esterase by the marine hydrocarbon degrading bacteria Alcanivorax borkumensis. The focus of this study is the remediation of petroleum hydrocarbons, hexane, hexadecane and motor oil as model substrates. A.

Impact of heterogeneous properties of soil and LNAPL on surfactant-enhanced capillary desaturation.

This paper investigates low-concentration (<1wt%) surfactant flushing when used as a follow-up technology for multiphase vacuum extraction on heterogeneous sites. Challenges posed by soil permeability, pore-size distribution, mineralogy, light non-aqueous phase liquid (LNAPL) weathering and groundwater hardness were quantified through batch and soil column tests. Compatibility issues between the mixed mineralogy soils, hard groundwater, mixed LNAPL and usual anionic surfactants were observed.

2D sandbox experiments of surfactant foams for mobility control and enhanced LNAPL recovery in layered soils.

The ability of surfactant foam to enhance mobility control and LNAPL recovery in a heterogeneous porous media was investigated through sandbox experiments with p-xylene used as LNAPL. A layered heterogeneous porous media was represented in a 2D sandbox filled with two layers of coarse and medium silica sand. Based on previous tests, the surfactant solution used was Ammonyx Lo at a concentration of 0.