Silver-Polymethylhydrosiloxane Nanocomposite Coating on Anodized Aluminum with Superhydrophobic and Antibacterial Properties.

Biofilm formation on both animate and inanimate surfaces serves as an ideal bacterial reservoir for the spread of nosocomial infections. Designing surfaces with both superhydrophobic and antibacterial properties can help reduce initial bacterial attachment and subsequent biofilm formation. In the present study, a two-step approach is deployed to fabricate silver-polymethylhydrosiloxane (Ag-PMHS) nanocomposites, followed by a simple dip-coating deposition on anodized Al.

Rapid concentration and molecular enrichment approach for sensitive detection of Escherichia coli and Shigella species in potable water samples.

In this work, we used a rapid, simple, and efficient concentration-and-recovery procedure combined with a DNA enrichment method (dubbed CRENAME [concentration and recovery of microbial particles, extraction of nucleic acids, and molecular enrichment]), that we coupled to an Escherichia coli/Shigella-specific real-time PCR (rtPCR) assay targeting the tuf gene, to sensitively detect E. coli/Shigella in water. This integrated method was compared to U.

Method for rapid and sensitive detection of Enterococcus sp. and Enterococcus faecalis/faecium cells in potable water samples.

We have developed a rapid and robust technological solution including a membrane filtration and dissolution method followed by a molecular enrichment and a real-time PCR assay, for detecting the presence of Enterococcus sp. or Enterococcus faecalis/faecium per 100 mL of water in less than 5 h and we compared it to Method 1600 on mEI agar in terms of specificity, sensitivity, and limit of detection. The mEI and the Enterococcus sp.

Analytical limits of three beta-glucosidase-based commercial culture methods used in environmental microbiology, to detect enterococci.

The enzyme-based test methods Enterolert, Chromocult Enterococci agar, and mEI agar, used to assess water quality through the detection Enterococcus spp., have been compared in terms of their analytical specificity and their ability to detect various enterococcal strains. To achieve this goal, we have tested 110 different non-enterococcal bacterial strains and 101 strains of Enterococcus spp.

2,6-Diphenylthiazolo[3,2-b][1,2,4]triazoles as telomeric G-quadruplex stabilizers.

The design and synthesis of 2,6-diphenylthiazolo[3,2-b][1,2,4]triazoles characterized by a large aromatic building block bearing cationic side chains are reported. These molecules are evaluated as telomeric G-quadruplex stabilizers and for their selectivity towards duplex DNA by competition experiments. Two compounds (14a, 19) were found active with high selectivity for telomeric G-quadruplex over duplex DNA.

Analytical limits of four beta-glucuronidase and beta-galactosidase-based commercial culture methods used to detect Escherichia coli and total coliforms.

Colilert (Colilert), Readycult Coliforms 100 (Readycult), Chromocult Coliform agar ES (Chromocult), and MI agar (MI) are beta-galactosidase and beta-glucuronidase-based commercial culture methods used to assess water quality. Their analytical performance, in terms of their respective ability to detect different strains of Escherichia coli and total coliforms, had never been systematically compared with pure cultures. Here, their ability to detect beta-glucuronidase production from E.

EPR study of copper(II) complexes of hydroxysalen derivatives in order to be used in the DNA cleavage.

Two series of functionalized hydroxy-salen-copper(II) complexes with various side chain lengths have been synthesized. The first one is characterised by amino side chain protected by the tert-butyloxycarbonyl group (Boc) whereas, the second series is obtained by removal of the Boc-protecting group under acidic conditions and formation instead of it an ammonium salt. EPR studies were carried out on the copper(II) complexes.

DNA modification by oxovanadium(IV) complexes of salen derivatives.

Oxovanadium(IV) complexes of hydroxysalen derivatives have been prepared and tested as DNA reactive agents. The nuclease activity has been investigated under oxidative or reducing conditions, on the basis of the various oxidation states of vanadium: V(III), V(IV) and V(V). In the absence of an activating agent, none of the compounds tested was able to induce cleavage of DNA, whereas in the presence of mercaptopropionic acid (MPA) or Oxone the four complexes induced DNA modifications.

Design, synthesis and glutathione peroxidase-like properties of ovothiol-derived diselenides.

Eleven imidazole diselenides derived from the naturally occurring family of antioxidants, the ovothiols, were synthetised by cyclisation of selenoamides with trimethylsilyltrifluoromethanesulfonate or refluxing of cyanoamines in a selenium/sodium borohydride mixture. These compounds were assayed for their glutathione peroxidase-like (GSH Px-like) activity and their capacity to be reduced by glutathione. The most active molecules of the series were 4 times more potent in the GSH Px-like test than the widely known reference compound, ebselen.

Potent mammalian cerebroprotection and neuronal cell death inhibition are afforded by a synthetic antioxidant analogue of marine invertebrate cell protectant ovothiols.

Implicit strategies for neuroprotection in the adult brain include GABAA receptor activation, N-methyl-d-aspartate receptor and sodium voltage-gated channel inhibition. Ironically, these same targets may be harmful to the immature or developing brain. Protection has been demonstrated for both immature and mature brain with the use of a synthetic ovothiol analogue.

Free radical production by hydroxy-salen manganese complexes studied by ESR and XANES.

Three salen-Mn(II) complexes bearing hydroxyl groups in either the ortho, para or meta positions have been synthesized and the structures of the metal complexes and their potential to produce free radicals investigated by electron spin resonance (ESR) and X-ray absorption near edge structures (XANES) spectroscopy. All three compounds were shown to generate a high level of superoxide anions in dimethyl sulfoxide (DMSO) solution. The production of oxygen radicals results from a one electron process oxidation of Mn(II) species leading to the formation Mn(III) redox state species, as revealed by a higher XANES edge energy of 2.