Publications by authors named "Jean-Francois Sternon"
Article Synopsis
- - The study focuses on a class III zoonotic bacterial pathogen that can survive and replicate within host cells, particularly macrophages, by using a detailed transposon sequencing analysis to identify essential genes.
- - Researchers created a comprehensive transposon mutant library and mapped nearly a million insertion sites, revealing 491 essential coding sequences, with chromosome I containing a higher percentage of essential genes than chromosome II.
- - They discovered 48 crucial genes for the bacteria's growth inside macrophages, highlighting the importance of the pyrimidine nucleotide biosynthesis pathway for its proliferation in these immune cells.
Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages.
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