Publications by authors named "Jean-Baptiste Bureau"
Article Synopsis
- - Adult human epidermal stem cells can be expanded outside the body for use in cell and gene therapy, but maintaining their "stemness" is crucial to prevent them from turning into less effective cells.
- - The study reveals that these stem cells can sense slight temperature changes, which affects signaling pathways in the cells, specifically through a process involving mTOR proteins.
- - Inhibiting a part of the mTOR signaling pathway (mTORC1) for a long time helps keep the cells as stem cells instead of converting them, which is significant for improving future regenerative medicine treatments.
Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality.
View Article and Find Full Text PDFThe impact of cellular individuality on host-microbe interactions is increasingly appreciated but studying the temporal dynamics of single-cell behavior in this context remains technically challenging. Here we present a microfluidic platform, InfectChip, to trap motile infected cells for high-resolution time-lapse microscopy. This approach allows the direct visualization of all stages of infection, from bacterial uptake to death of the bacterium or host cell, over extended periods of time.
View Article and Find Full Text PDF