How to Conduct Phylogenetic Endoglucanase () Inference Using the Reference Species Complex Curated Database?

The phytopathogenic species complex (RSSC) was recently divided into three distinct species, after long-standing researches. About twenty years ago, phylotype-based classification was introduced to mark the beginning of DNA-based taxonomy within RSSC. Within each phylotype, the "sequevar" classification further refines subspecies designations, based on variations in the endoglucanase () gene sequence, and these sequevars are unique to each phylotype.

Molecular Epidemiology of Phylotype I Strains in the Southwest Indian Ocean Region and Their Relatedness to African Strains.

The increasing requirement for developing tools enabling fine strain traceability responsible for epidemics is tightly linked with the need to understand factors shaping pathogen populations and their environmental interactions. Bacterial wilt caused by the species complex (RSSC) is one of the most important plant diseases in tropical and subtropical regions. Sadly, little, outdated, or no information on its epidemiology is reported in the literature, although alarming outbreaks are regularly reported as disasters.

Sequevar Diversity and Virulence of Phylotype I on Mayotte Island (Indian Ocean).

The genetic and phenotypic diversity of the species complex, which causes bacterial wilt to Solanacae, was assessed in 140 strains sampled from the main vegetable production areas of the Mayotte island. Only phylotype I strains were identified in the five surveyed areas. The strains were distributed into the following 4 sequevars: I-31 (85.

Genetic Diversity of the Species Complex in the Southwest Indian Ocean Islands.

Epidemiological surveillance of plant pathogens based on genotyping methods is mandatory to improve disease management strategies. In the Southwest Indian Ocean (SWIO) islands, bacterial wilt (BW) caused by the species complex (RSSC) is hampering the production of many sustainable and cash crops. To thoroughly analyze the genetic diversity of the RSSC in the SWIO, we performed a wide sampling survey (in Comoros, Mauritius, Reunion, Rodrigues, and Seychelles) that yielded 1,704 isolates from 129 plots, mainly from solanaceous crops.

Evaluation of procedures for reliable PCR detection of Ralstonia solanacearum in common natural substrates.

Several procedures were compared for reliable PCR detection of Ralstonia solanacearum in common substrates (plant, seed, water and soil). In order to prevent the inhibition of PCR by substances contained in crude extracts, numerous DNA extraction procedures as well as additives to buffers or PCR mixtures were checked. Our results showed that the efficiency of these methods or compounds depended greatly upon the nature of the sample.