Cytoplasmic actin A3 gene promoter injected as supercoiled plasmid is transiently active inBombyx mori embryonic vitellophages.

Freshly deposited eggs ofBombyx mori were microinjected with supercoiled plasmid DNA which carried the β-galactosidase coding sequence ofEscherichia coli inserted in place of the coding sequence of theB. mori cytoplasmic actin A3 gene. Transient expression of this fusion gene in the embryo was determined by in situ histochemical detection of enzyme activity.