Enhancement of glucose uptake in muscular cell by soybean charged peptides isolated by electrodialysis with ultrafiltration membranes (EDUF): activation of the AMPK pathway.

Soy peptides consumption has been associated with beneficial effects in type 2 diabetes patients. However, the peptide fractions responsible for these effects, and their mechanisms of action, have not been identified yet. In this study, we have isolated soybean peptides by electrodialysis with an ultrafiltration membrane (EDUF) at 50 V/100 kDa, and tested them for their capacity to improve glucose uptake in L6 muscle cells.

Impact of feed solution flow rate on Peptide fractionation by electrodialysis with ultrafiltration membrane.

Recently, processes combining an electrical field as a driving force to porous membranes have been developed for the separation of protein or peptide mixtures to obtain more purified products with higher functionality or nutritional value. The objective of this work was to evaluate the influence of the flow rate on the productivity and selectivity as well as on the electrodialytic parameters of electrodialysis with an ultrafiltration membrane (EDUF) during the fractionation of peptides from a beta-lactoglobulin tryptic hydrolysate. It appeared that the feed solution flow rate had no impact on the yield of the process but induced changes in the selectivity.

Simultaneous separation of acid and basic bioactive peptides by electrodialysis with ultrafiltration membrane.

beta-Lactoglobulin (beta-lg), one of the major whey components, can release by enzymatic hydrolysis different bioactive peptidic sequences according to the enzyme used. However, these protein hydrolysates have to be fractionated to obtain peptides in a more purified form. The aim of the present work was to evaluate the feasibility of separating peptides from a beta-lg hydrolysate using an ultrafiltration (UF) membrane stacked in an electrodialysis (ED) cell and to study the effect of pH on the migration of basic/cationic and acid/anionic peptides in the ED configuration.

Effects of type of added salt and ionic strength on physicochemical and functional properties of casein isolates produced by electroacidification.

A procedure developed for soybean protein precipitation which was based on electrodialysis was tested for the production of acid casein from reconstituted skim milk. In a previous paper, the performance of bipolar membrane electroacidification (BMEA) was evaluated under different conditions of ionic strength (micro(added) = 0, 0.25, 0.

Identification of Skim Milk Electroacidification Fouling: A Microscopic Approach.

Bipolar membrane electroacidification (BMEA) was used to produce high-purity bovine milk casein isolates. However, a deposit suggested to be a mix of calcium and magnesium salts was observed on the cation-exchange membrane (CEM) side in contact with the base. The aim of the present study was to complete the identification of the deposit formed on CMX membrane during bipolar membrane electroacidification of skim milk, and to characterize its physical structure at the interfaces of CEM.