Cercosporamide, a polyketide-derived fungal metabolite, serves as an antifungal agent against phytopathogenic fungi.

An endophytic fungus, sp. NG-25, produces a set of structurally related polyketides including cercosporamide, phomodione, and usnic acid, among which, cercosporamide has been reported to have strong antifungal and anticancer activities. In this study, sp.

Establishment of -Mediated Transformation of , a Model Lichen-Forming Fungus.

Despite the fascinating biology of lichens, such as the symbiotic association of lichen-forming fungi (mycobiont) with their photosynthetic partners and their ability to grow in harsh habitats, lack of genetic tools manipulating mycobiont has hindered studies on genetic mechanisms underpinning lichen biology. Thus, we established an -mediated transformation (ATMT) system for genetic transformation of a mycobiont isolated from . A set of combinations of ATMT conditions, such as input biomass of mycobiont, co-cultivation period with cells, and incubation temperature, were tested to identify an optimized ATMT condition for the mycobiont.