Molecular characterization, haplotype analysis and development of markers specific to gene regulating methionine accumulation in kernels of subtropical maize.

Unlabelled: Maize kernel protein is deficient in sulfur-containing essential amino acid such as methionine. The gene encodes methionine-rich 18-kDa δ-zein in maize kernels. In this study, we sequenced full-length of gene (820 bp) among 10 maize inbreds, revealing 43 SNPs and 22 (average length-7.

Allelic variation and haplotype diversity of () gene governing in vivo maternal haploid induction in maize.

Unlabelled: Diverse haploid inducer lines with > 6% of haploid induction rate are now routinely used to develop doubled haploid lines. Though gene regulates haploid induction, its molecular characterization and haplotype analysis in maize and its related species have not been undertaken so far. In the present study, the entire 1812 bp long gene was sequenced among two mutant and eight wild-type inbreds.

Starch and storage protein dynamics in the developing and matured grains of durum wheat and diploid progenitor species.

Durum wheat, less immunogenically intolerant than bread wheat, originates from diploid progenitors known for nutritional quality and stress tolerance. Present study involves the analysis of major grain parameters, viz. size, weight, sugar, starch, and protein content of Triticum durum (AABB genome) and its diploid progenitors, Triticum monococcum (AA genome) and Aegilops speltoides (BB genome).

Variation in anthocyanin pigmentation by R1-navajo gene, development and validation of breeder-friendly markers specific to C1-Inhibitor locus for in-vivo haploid production in maize.

Background: In-vivo maternal haploids serve as the basis of doubled haploid (DH) breeding in maize. R1-navajo (R1-nj) gene governing anthocyanin colouration in the endosperm and embryo is widely used to identify haploid seeds. However, the expression of R1-nj depends on genetic-background of source-germplasm used for deriving DH-lines.

Identification of putative flowering genes and transcription factors from flower transcriptome dataset of tuberose ( L.).

is commercially popular because of their economic importance in floriculture for cut and loose flowers and in perfume industry because of the unique fragrance. Despite its commercial importance, no ready-to-use transcript sequence information is available in the public database. We have sequenced the RNA obtained from tuberose flowers using the Illumina HiSeq.

In vitro somatic embryogenesis and plantlet regeneration from immature male inflorescence of adult dura and tenera palms of Elaeis guineensis (Jacq.).

We report here a method for plant regeneration through somatic embryogenesis from explants collected from immature male inflorescence of adult oil palm cultivated in India. Callus induction was successful from tissues of immature male inflorescence collected from both dura and tenera varieties of oil palm. A modified Y3 (Eeuwens) media supplemented with several additives and activated charcoal (3%) were used for the experiments.