Determination of Acephate, Dinotefuran, and Emamectin Benzoate in a Pesticide Formulation: A Single-Laboratory Validation.

Background: The advantage of simultaneous separation and quantification is the reduction of analysis time and consumption of solvents and reagents.

Objective: The objective of the present investigation was to optimize and validate a novel, rapid, and simple reverse-phase high-performance liquid chromatographic method for the simultaneous determination of acephate, dinotefuran, and emamectin benzoate in a pesticide formulation.

Method: The chromatographic separation and quantification were accomplished by using Kromasil CN column (250 mm × 4.