Author Correction: Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Cell Cycle Regulation of the Centrosome and Cilium.

Centrosomes and cilia are conserved microtubule-based organelles whose structure and function depend on cell cycle stages. In dividing cells, centrosomes organize mitotic spindle poles, while in differentiating cells, centrosomes template ciliogenesis. Classically, this functional dichotomy has been attributed to regulation by cell cycle-dependent post-translational modifications, and recently PLK1, Nek2, Aurora A, and tubulin deacetylase were implicated in regulating the transition from cilia to centrosome.

Building a centriole.

Centrioles are the key foundation of centrosomes and cilia, yet a molecular understanding of how they form has only recently begun to emerge. Building a fully functional centriole that can form a centrosome and cilium requires two cell cycles. Centriole building starts with procentriole nucleation, a process that is coordinated by the conserved proteins Plk4/Zyg-1, and Asterless/Cep152.

Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment.

Regulated centrosome biogenesis is required for accurate cell division and for maintaining genome integrity. Centrosomes consist of a centriole pair surrounded by a protein network known as pericentriolar material (PCM). PCM assembly is a tightly regulated, critical step that determines the size and capability of centrosomes.

Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome.

Centrosomes are conserved organelles that are essential for accurate cell division and cilium formation. A centrosome consists of a pair of centrioles surrounded by a protein network of pericentriolar material (PCM) that is essential for the centrosome's function. In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole.

Self-assembling SAS-6 multimer is a core centriole building block.

Centrioles are conserved microtubule-based organelles with 9-fold symmetry that are essential for cilia and mitotic spindle formation. A conserved structure at the onset of centriole assembly is a "cartwheel" with 9-fold radial symmetry and a central tubule in its core. It remains unclear how the cartwheel is formed.

Drosophila asterless and vertebrate Cep152 Are orthologs essential for centriole duplication.

The centriole is the core structure of centrosome and cilium. Failure to restrict centriole duplication to once per cell cycle has serious consequences and is commonly observed in cancer. Despite its medical importance, the mechanism of centriole formation is poorly understood.

Studies on hydrogenase activity and chlorobenzene respiration in Dehalococcoides sp. strain CBDB1.

Hydrogen oxidation and electron transport were studied in the chlorobenzene-utilizing anaerobe Dehalococcoides sp. strain CBDB1. While Cu(2+) and Hg(2+) ions irreversibly inhibited hydrogenase activity in intact cells, Ni(2+) ions inhibited reversibly.

Dehalorespiration with hexachlorobenzene and pentachlorobenzene by Dehalococcoides sp. strain CBDB1.

The chlororespiring anaerobe Dehalococcoides sp. strain CBDB1 used hexachlorobenzene and pentachlorobenzene as electron acceptors in an energy-conserving process with hydrogen as electron donor. Previous attempts to grow Dehalococcoides sp.