Impact of in vitro lens deposition and removal on bacterial adhesion to orthokeratology contact lenses.

Purpose: The purpose of this study was to explore the impact of several contact lens (CL) care solutions on the removal of proteins and lipids, and how deposit removal impacts bacterial adhesion and solution disinfection.

Methods: Lysozyme and lipid deposition on three ortho-k (rigid) and two soft CL materials were evaluated using an ELISA kit and gas chromatography respectively. Bacterial adhesion to a fluorosilicone acrylate material using Pseudomonas aeruginosa with various compositions of artificial tear solutions (ATS), including with denatured proteins, was also investigated.

Short-term tolerability of commercial eyelid cleansers: A randomised crossover study.

Purpose: To evaluate the short-term tolerability of five commercially available anti-demodectic eyelid cleansers; OCuSOFT Oust Demodex (OD), I-MED I-Lid'n Lash Plus (ILL+), Labtician BlephaDex (BD), Chrissanthe Eye Cleanse (EC), and Théa Blephademodex (BDdx).

Methods: Thirty healthy non-contact lens wearers (18 female; mean ± SD age, 33 ± 12 years) were enrolled in a prospective randomised crossover study. On separate visits, spaced at least 48 h apart, participants were randomised to receive topical application of one of five eyelid cleansers or saline.

Optimization of goblet cell density quantification methods.

The purpose of this study was to develop a standardized, accurate and efficient method for estimating conjunctival goblet cell density (GCD) via optimizing sample storage conditions and quantification methods. Conjunctival impression cytology (CIC) membranes were collected from both eyes of 32 participants and were randomized to two storage durations (2-3 weeks, 6-7 weeks) and two storage container types (microcentrifuge tube, flat histology cassette). The CIC membranes were stained and subdivided into 25 areas (5 mm × 5 mm) for imaging and the GCs were counted under 200X magnification using three different methods: (1) full CIC membrane GC count of the 25 images with cell-counting software ("full"; reference method), (2) partial membrane GC count of 9 images with cell-counting software ("partial"), and (3) manual counting of the 25 images ("manual").

Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation.

Artificial lipid-containing tear formulations are developed to reduce tear evaporation by the restoration of a deficient tear lipid layer. Artificial tear formulations that prevent cell desiccation will result in ocular surface protection and the maintenance of cell metabolic activity. During dehydration, cells undergo the process of loss of metabolic activity and subsequently cell death.

Adhesion of , , , to contact lenses under the influence of an artificial tear solution.

Corneal infection is a devastating sight-threatening complication that is associated with contact lens (CL) wear, commonly caused by . Lately, , , and have been associated with corneal infection. This study investigated the adhesion of these emerging pathogens to CLs, under the influence of an artificial tear solution (ATS) containing a variety of components commonly found in human tears.

Microbial Contamination of Contact Lens Storage Cases During Daily Wear Use.

Purpose: To evaluate contact lens (CL) storage case contamination when used with four different CL care solutions during daily wear of three different CL materials.

Methods: A parallel, prospective, bilateral, randomized clinical trial (n = 38) was conducted. Subjects were randomly assigned to use one of three CL materials (etafilcon A, senofilcon A, or galyfilcon A) on a daily wear basis.

In vivo assessment of antimicrobial efficacy of silver-impregnated contact lens storage cases.

Purpose: To evaluate microbial contamination in silver-impregnated contact lens (CL) storage cases while establishing the effect of "wet " and "dry " case maintenance and to determine its association with clinical signs, symptoms, and compliance.

Methods: Two noncontemporaneous prospective studies were conducted. Regular storage cases in study 1 (n = 40) and silver-impregnated cases in study 2 (n = 41) were used in conjunction with CL solution and CLs (balafilcon A).

Biocidal efficacy of silver-impregnated contact lens storage cases in vitro.

Purpose: Silver-impregnated contact lens (CL) storage cases are designed to reduce microbial contamination during use, but there are limited data on their effectiveness. This study evaluated early antimicrobial activity of silver-impregnated CL cases and silver-release characteristics in vitro.

Methods: Three silver-impregnated CL storage cases-MicroBlock (CIBA Vision, Atlanta, GA), i-clean (Sauflon Pharmaceuticals Ltd.