The Role of Cytonemes and Diffusive Transport in the Establishment of Morphogen Gradients.

Spatial distributions of morphogens provide positional information in developing systems, but how the distributions are established and maintained remains an open problem. Transport by diffusion has been the traditional mechanism, but recent experimental work has shown that cells can also communicate by filopodia-like structures called cytonemes that make direct cell-to-cell contacts. Here we investigate the roles each may play individually in a complex tissue and how they can jointly establish a reliable spatial distribution of a morphogen.

The effects of internal forces and membrane heterogeneity on three-dimensional cell shapes.

The shape of cells and the control thereof plays a central role in a variety of cellular processes, including endo- and exocytosis, cell division and cell movement. Intra- and extracellular forces control the shapes, and while the shape changes in some processes such as exocytosis are intracellularly-controlled and localized in the cell, movement requires force transmission to the environment, and the feedback from it can affect the cell shape and mode of movement used. The shape of a cell is determined by its cytoskeleton (CSK), and thus shape changes involved in various processes involve controlled remodeling of the CSK.

A Random Walk Approach to Transport in Tissues and Complex Media: From Microscale Descriptions to Macroscale Models.

The biological processes necessary for the development and continued survival of any organism are often strongly influenced by the transport properties of various biologically active species. The transport phenomena involved vary over multiple temporal and spatial scales, from organism-level behaviors such as the search for food, to systemic processes such as the transport of oxygen from the lungs to distant organs, down to microscopic phenomena such as the stochastic movement of proteins in a cell. Each of these processes is influenced by many interrelated factors.

Growth control in the Drosophila wing disk.

The regulation of size and shape is a fundamental requirement of biological development and has been a subject of scientific study for centuries, but we still lack an understanding of how organisms know when to stop growing. Imaginal wing disks of the fruit fly Drosophila melanogaster, which are precursors of the adult wings, are an archetypal tissue for studying growth control. The growth of the disks is dependent on many inter- and intra-organ factors such as morphogens, mechanical forces, nutrient levels, and hormones that influence gene expression and cell growth.