The COVID-19 pandemic continues to be a concern and keeps global health authorities on alert. The RT-PCR technique has been the gold-standard assay for detecting the SARS-CoV-2 virus. However, rapid antigen tests (RATs) have been widely used to increase the number of tests faster and more efficiently in the population.
View Article and Find Full Text PDFIn this work, coaxial conductor-ceramic direct ink writing enables the printing of sensitive or encapsulated materials onto heterogeneous and rough substrates. While encasing the core fluid within a stiff ceramic shell, continuity may be maintained, even while printing onto conventionally challenging substrates. Here, we report the development of a coaxial ceramic direct ink writing suite and explore coflow interrelationships based on microfluidic principles.
View Article and Find Full Text PDFCancer of the reproductive tract includes diseases with higher prevalence in the female population. This investigation examined whether an anthocyanin-enriched extract of , commonly known as "maqui," could affect some hallmarks of endometrial cancer. Cultures of the human endometrial cancer cell line Ishikawa were treated with a hydroethanolic maqui extract at 1, 3, 10, 30, 100, 300, or 1000 µg/mL to determine the effect on cell viability by MTT assay.
View Article and Find Full Text PDFWe analysed whether the hydroethanolic extracts from leaves of Remy (bailahuen) and Palau (cedron) inhibit the growth and ability of Enteritidis to form biofilms and to adhere to human intestinal epithelial cells. Herein, we first determined the total phenolic content and antioxidant and antibacterial activities of the extracts. Then, Enteritidis was treated with the extracts to analyse biofilm formation by scanning electronic microscopy and the violet crystal test.
View Article and Find Full Text PDFWhole dead tumor cells can be used as antigen source and the induction of protective immune response could be enhanced by damage-associated molecular patterns. We generated whole dead tumor cells called B16-immunogenic cell bodies (ICBs) from B16 melanoma cells by nutrient starvation and evaluated the antitumor effect of B16-ICBs plus ATP and polymyxin B (PMB). The subcutaneous immunization with B16-ICBs + PMB + ATP a 50% of tumor-free animals and induced a significant delay in tumor growth in a prophylactic approach.
View Article and Find Full Text PDFMelanoma immunotherapy, specifically the autotransplant of dendritic cells charged with tumors antigens, has shown promising results in clinical trials. The positive clinical effects of this therapy have been associated to increased Th17 response and delayed-type hypersensitivity (DTH) against to tumor antigens. Some synthetic compounds, such as diphenylcyclopropenone (DPCP), are capable of triggering a DTH response in cutaneous malignancies and also to induce clinically relevant effects against melanoma.
View Article and Find Full Text PDFThe metabolite 2-methoxyestradiol (2ME) is an endogenous estrogen metabolite with potential therapeutic properties in reproductive cancers. However, the molecular mechanisms by which 2ME exerts its anticancer activity are not well elucidated. The purpose of this study was to determine the molecular signals associated with the apoptotic effects of 2ME in a human endometrial cancer cell line.
View Article and Find Full Text PDFThis paper presents the Constructed Wetland Model No1 (CWM1), a general model to describe biochemical transformation and degradation processes for organic matter, nitrogen and sulphur in subsurface flow constructed wetlands. The main objective of CWM1 is to predict effluent concentrations from constructed wetlands without predicting gaseous emissions. CWM1 describes aerobic, anoxic and anaerobic processes and is therefore applicable to both horizontal and vertical flow systems.
View Article and Find Full Text PDFNumerical modelling of subsurface flow constructed wetlands (CWs) gained increasing interest during the last years. The main objective of the modelling work is, on the one hand, to increase the insight in dynamics and functioning of the complex CW system by using mechanistic or process based models that describe transformation and degradation processes in detail. As these mechanistic models are complex and therefore rather difficult to use there is, on the other hand, a need for simplified models for CW design.
View Article and Find Full Text PDFA poodle was admitted for investigation of pruritus and a lesional pattern of erythema and alopecia located in the dorsolumbar area. After differential diagnosis ruling out several processes a rare side effect to furosemide, not yet described in canine medicine was confirmed as the possible causative agent.
View Article and Find Full Text PDF