Effect of autologous transplant of peripheral blood mononuclear cells in combination with proangiogenic factors during experimental revascularization of lower limb ischemia.

Peripheral blood mononuclear cells (PBMCs) contain a cell fraction of mononuclear progenitor cells (MPCs), which own significant angiogenic potential. Autologous transplant of PBMC and/or platelet-rich plasma (PRP) promotes endothelial cells differentiation in experimental lower limb ischemia, which is considered a safe and effective strategy to support revascularization, either in animal models or clinical trials. In addition, thrombin has been proposed to enrich biological scaffolds, hence increasing MPC viability after intramuscular administration, whereas proangiogenic mediators such as vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNF-α), inhibitor of the plasminogen activator-1 (PAI-1), and chemokine (CXCL1; GRO-α) participate in the endothelial response to ischemia, through their proangiogenic effects over endothelial cells proliferation, survival, migration, endothelial integrity maintenance, and physiologic vascular response to injury.

[Vascular microsurgery course: 40 years at the Centro Médico Nacional 20 de Noviembre (ISSSTE) and 33 years at the Faculty of Medicine of the UNAM].

On June 1977, "Centro Médico Nacional 20 de Noviembre," in Mexico City, implemented the first vascular microsurgery course. The aim was to develop clinical applications of microsurgery focus on surgical specialties, due to the necessity to develop microsurgical skills. On August 1964 we started the program course at the surgical department of the School of Medicine at Universidad Nacional Autónoma de México (UNAM).

Single-port endo-laparoscopic surgery in combined abdominal procedures.

Single-port endo-laparoscopic surgery has gained support in the surgical community because it is perceived to offer a better postoperative outcome as it requires only a single incision. We write this prospective observational study to ascertain the feasibility and safety of this technique in patients otherwise requiring two operations. Five patients who underwent double procedures with a single-port device were reviewed: Case 1, a transabdominal preperitoneal hernia repair and gastric wedge resection; Case 2, cholecystectomy and diaphragmatic hernia repair; Case 3, oophorectomy and incisional hernia repair; Case 4, anterior resection of the rectum and hepatic segmentectomy; and Case 5, left adrenalectomy and cholecystectomy.

[Prevalence of Barrett's esophagus in non-selected patients submitted to esophagogastroduodenoscopy and associated risk factors].

Objective: To establish the prevalence of the Barrett esophagus (BE) in non-selected patients who underwent upper gastrointestinal endoscopy (UGE) and identified risk factors associated.

Methods: Case-control study on patients who underwent UGE between 2001-2003. Demographic data was gathered as well as the presence of esophagitis, H.