Multimodal Membrane Poration by Thanatin.

Antimicrobial resistance has motivated the search for antimicrobial agents with multimodal mechanisms of action. Host defense peptides and bacteriocins hold particular promise in this regard. Among many molecules discovered to date, thanatin appears to represent the properties of the two classes in that it, like bacteriocins, adopts a highly stable fold in solution and, like host defense peptides, exhibits broad-spectrum antibiotic activity.

DANAMIC: Data analyzer of minimum inhibitory concentrations - Protocol to analyze antimicrobial susceptibility data.

This protocol describes an open-source software developed to analyze experimental data obtained using antimicrobial susceptibility assays. We first describe experimental procedures for testing the activity of antimicrobial agents based on reference standards (BS EN ISO 20776-1:2020). We then describe the software protocol to analyze and convert the data obtained using these procedures into minimum inhibitory concentrations.