Rational Design of Live Biotherapeutic Products for the Prevention of Clostridioides difficile Infection.

Clostridioides difficile infection (CDI) is a major cause of healthcare- and antibiotic-associated diarrhea. While fecal microbiota transplantation (FMT) shows promise for recurrent CDI, its mechanisms and long-term safety are not fully understood. Live biotherapeutic products (LBPs) using pre-defined bacterial consortia offer an alternative option, but the rational designing LBPs remains challenging.

Absence of Toxemia in Clostridioides difficile Infection: Results from Ultrasensitive Toxin Assay of Serum.

Clostridioides difficile infection (CDI) is caused by Toxins A and B, secreted from pathogenic strains of C. difficle. This infection can vary greatly in symptom severity and in clinical presentation.

Involvement of the GABAergic system in the neuroprotective and sedative effects of acacetin 7-O-glucoside in rodents.

Purpose: Characterization of sedative, possible anticonvulsant, and protective effects of Acacetin-7-O-glucoside (7-ACAG).

Methods: 7-ACAG was separated and its purity was analyzed. Its sedative and anti-seizure effects (1, 10, 20, and 40 mg/kg) were evaluated in male mice.

Repeated-High-Intensity-Running Activity and Internal Training Load of Elite Rugby Sevens Players During International Matches: A Comparison Between Halves.

Purpose: To describe the repeated-high-intensity activity and internal training load of rugby sevens players during international matches and to compare the differences between the 1st and 2nd halves.

Methods: Twelve international-level male rugby sevens players were monitored during international competitive matches (n = 30 match files) using global positioning system technology and heart-rate monitoring.

Results: The relative total distance covered by the players throughout the match was 112.

Concurrent repeated-sprint and resistance training with superimposed vibrations in rugby players.

Purpose: To examine the effect of repeated-sprint training (RST) vs combined RST and resistance training with superimposed vibrations on repeated-sprint ability (RSA) and lower-body power output in male rugby players.

Methods: Players were divided into 2 training groups. One group performed RST (n = 10) 2 d/wk and the other performed RST 1 d/wk and squat resistance training with superimposed vibrations on the second day (RS+ST; n = 10).

Treatment with human metalloproteinase-8 gene delivery ameliorates experimental rat liver cirrhosis.

Background & Aims: An extrahepatic human neutrophil collagenase complementary DNA (matrix metalloprotease-8) cloned in an adenovirus vector was used as a therapeutic agent in cirrhosis.

Methods: A high titer of clinical-grade AdMMP8 was obtained.

Results: HeLa cells transduced with AdMMP8 expressed recombinant matrix metalloprotease-8 messenger RNA and matrix metalloprotease-8 protein.

Improved effects of viral gene delivery of human uPA plus biliodigestive anastomosis induce recovery from experimental biliary cirrhosis.

Gene therapy may represent a new avenue for the development of multimodal treatment for diverse forms of cirrhosis. This study explores the potential benefits of combining adenovirus-mediated human urokinase-plasminogen activator (AdHuPA) gene delivery and biliodigestive anastomosis to enhance the therapeutic efficacy of each treatment alone for cholestatic disorders resulting in secondary biliary cirrhosis. In an experimental model of secondary biliary cirrhosis, application of 6 x 10(11) vp/kg AdHuPA adenovirus vector resulted in 25.