Publications by authors named "Javier Enciso"
Stem cell therapy in dogs has increased considerably in recent years; however, there are few publications on the treatment of perianal fistulas (PF) in this species. The aim of this open-label clinical trial was to demonstrate the efficacy and safety of a new protocol for the in situ administration of low-dose adipose-derived allogeneic stem cells (ASCs) for the treatment of refractory spontaneous perianal fistula. The methodology consisted of applying one to three doses of 5 × 10 cryopreserved allogeneic ASCs to each fistula.
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates the relationship between CD44 and CD63 protein expressions and various clinicopathological features in breast cancer, including tumor-infiltrating lymphocytes, specific genetic mutations, and patient survival rates.
- - Findings from 101 breast cancer samples reveal that high CD44 expression is linked to younger age, a triple-negative cancer type, and shorter survival, while high CD63 density correlates with estrogen receptor positivity and lower tumor-infiltrating lymphocyte levels.
- - The results suggest that CD44 is associated with more aggressive cancer characteristics, while lower CD63 density is observed in tumors with aggressive traits.
Canine parvovirus is a highly contagious pathogen affecting domestic dogs and other carnivores globally. Monitoring CPV through continuous genomic surveillance is crucial for mapping variability and developing effective control measures. Here, we developed a method using multiplex-PCR-next-generation sequencing to obtain full-length CPV genomes directly from clinical samples.
View Article and Find Full Text PDFModel of Liver Fibrosis Induction by Thioacetamide in Rats for Regenerative Therapy Studies.
Anal Cell Pathol (Amst)
November 2022
Hepatic fibrosis is caused by chronic injury due to toxic, infectious, or metabolic causes, and it may progress to cirrhosis and hepatocellular carcinoma. There is currently no antifibrotic therapy authorized for human use; however, there are promising studies using cell therapies. There are also no animal models that exactly reproduce human liver fibrosis that can be used to better understand the mechanisms of its regression and identify new targets for treatment and therapeutic approaches.
View Article and Find Full Text PDFObjective: To evaluate the relationship between circulating tumor DNA (ctDNA) presence and tumor features including tumor-infiltrating lymphocyte (TIL) levels in Peruvian breast cancer patients.
Materials And Methods: This was a prospective study conducted at the Instituto Nacional de Enfemedades Neoplasicas, Peru. We evaluated level of TIL and PIK3CA mutations in ctDNA.
Background And Aim: Ribonucleic acid viruses remain latent in different cell types, including mesenchymal stem cells; however, the distemper virus remains undetected in these cells. This study aimed to determine whether adipose stem cells (ASCs) from dogs with distemper disease are infected with the canine morbillivirus (CM).
Materials And Methods: Twelve dogs with the neurological phase of the disease and who were positive for CM by reverse transcription polymerase chain reaction (RT-PCR), were studied.
Triple-negative breast tumours (TNBTs) make up 15-20% of all breast tumours. There is no treatment for them, and the role that cancer stem cells (CSCs) have in carcinogenesis is still unclear, so finding markers and therapeutic targets in CSC exosomes requires these cells to exist as a homogeneous cell population. The objective of this work was to determine differences in ultrastructural morphology, proliferative capacity, and mouse-xenotransplantation characteristics of the MDA-MB-231 and MDA-MB-436 TNBT cell lines with the CD44 /CD24 phenotype in order to study their exosomes.
View Article and Find Full Text PDFAim: The aim of this pilot study was to evaluate the therapeutic and safety performance of an intramuscular treatment protocol of multidose of allogeneic adipose stem cells (ASCs) isolated, characterized, and expanded from a healthy canine donor.
Materials And Methods: Twelve dogs diagnosed with canine atopic dermatitis (CAD) were intramuscularly treated with 0.5×10 of cryopreserved ASCs from a healthy immunized young canine free donor weekly for 6 weeks.