Publications by authors named "Javier E Jimenez-Salazar"

Purpose: The epithelial-to-mesenchymal transition (EMT) is the main event that favors cell migration and metastasis in breast cancer. Previously, we demonstrated that 1 nM estradiol (E) promotes EMT, induced by c-Src kinase, causing changes in the localization of proteins that compose the tight junction (TJ) and adherens junction (AJ).

Methods: The present work highlights the central role of c-Src in the initiation of metastasis, induced by E, through increasing the ability of MCF-7 and T47-D cells, which express estrogen receptor alpha (ERα), to migrate and invade before they become metastatic.

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Estrogens have been implicated in the etiology of breast cancer for a long time. It has been stated that long-term exposure to estrogens is associated with a higher incidence of breast cancer, since estradiol (E) stimulates breast cell growth; however, its effect on DNA damage/repair is only starting to be investigated. Recent studies have documented that estrogens are able to modify the DNA damage response (DDR) and DNA repair mechanisms.

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In the present study, we investigated the effects of 17β-estradiol (E) on membrane roughness and gold nanoparticle (AuNP) uptake in MCF-7 breast cancer cells. Estrogen receptor (ER)-positive breast cancer cells (MCF-7) were exposed to bare 20 nm AuNPs in the presence and absence of 1×10 M E for different time intervals for up to 24 hrs. The effects of AuNP incorporation and E incubation on the MCF-7 cell surface roughness were measured using atomic force microscopy (AFM).

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Article Synopsis
  • GDF11 is identified as a crucial factor in regulating cell differentiation and has been poorly studied in cancer, especially in hepatocellular carcinoma (HCC), an aggressive liver cancer.
  • Treatment with GDF11 reduced the proliferation and colony forming ability of HCC cell lines, altering key cell cycle proteins and indicating compromised cell functionality.
  • Additionally, GDF11 treatment led to long-lasting effects on self-renewal capacity and significantly reduced cell migration and proliferation in vivo, suggesting its potential as a tumor suppressor in HCC.
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Biosensor technology has great potential for the detection of cancer through tumor-associated molecular biomarkers. In this work, we describe the immobilization of the recombinant humanized anti-HER2 monoclonal antibody (trastuzumab) on a silver nanostructured plate made by pulsed laser deposition (PLD), over a thin film of Au(111). Immobilization was performed via 4-mercapto benzoic acid self-assembled monolayers (4-MBA SAMs) that were activated with coupling reagents.

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Tumor cells utilize inappropriate epithelial-mesenchymal transition (EMT) mechanisms during the invasive process. It is becoming increasingly clear that estradiol (E2) induces breast cancer cell progression and enhances EMT; however, the mechanisms associated with this are unclear. We investigated the role of E2 on the expression and intracellular localization of the tight junction (TJ)-associated proteins, zonula occluden 1 (ZO-1), ZO-1-associated nucleic acid binding (ZONAB), and occludin, on the activation of c-Src and human epidermal growth factor receptor 2 (HER2) expression and cellular migration in the estrogen receptor (ER)-positive breast cancer cell lines, MCF-7 and T47D.

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