Cattle infection response network and its functional modules.

Background: Weighted Gene Co-expression Network analysis, a powerful technique used to extract co-expressed gene pattern from mRNA expression data, was constructed to infer common immune strategies used by cattle in response to five different bacterial species (Escherichia coli, Mycobacterium avium, Mycobacterium bovis, Salmonella and Staphylococcus aureus) and a protozoa (Trypanosoma Congolense) using 604 publicly available gene expression microarrays from 12 cattle infection experiments.

Results: A total of 14,999 transcripts that were differentially expressed (DE) in at least three different infection experiments were consolidated into 15 modules that contained between 43 and 4441 transcripts. The high number of shared DE transcripts between the different types of infections indicated that there were potentially common immune strategies used in response to these infections.

Cloprostenol injection improves reproductive characteristics in low libido Iranian Holstein bulls.

The objective of this study was to determine whether injections of Cloprostenol (PGF2alpha analog) in low libido Holstein bulls can improve seminal characteristics and libido. Ten low libido Iranian Holstein bulls were randomly assigned to two groups and received; (1) 250 microg ofCloprostenol (n=5) or (2) 2 mL of saline (n=5) 30 min prior to collection of semen 2 days per week for 2 months. Reaction time was significantly decreased in treatment group.

Polymorphism of the bovine POU1F1 gene: allele frequencies and effects on milk production in three Iranian native breeds and Holstein cattle of Iran.

The aim of this study was to estimate the allele frequencies in polymorphic site of exon six of POU1F1 gene in three Iranian native and Holstein cattle. Genomic DNA was extracted from 3 Iranian native cattle breeds, including 97 Mazandarani, 87 Sarabi, 112 Golpaygani and also 110 Holstein cattle. A 451 bp fragment of intron 5 and exon 6 were amplified and digested with HinfI restriction enzyme.

Evaluation of eight microsatellite loci polymorphism in four Japanese quail (Coturnix japonica) strain in Iran.

Allele frequencies of eight microsatellite loci as GUJ0001, GUJ0021, GUJ0034, GUJ0041, GUJ0049, GUJ0059, GUJ0070 and GUJ0097 was estimated for four strain of Japanese quail in Iran. Whole blood samples were collected from 200 individuals belonging to four strain (Pharach, Panda, Tuxedo and Golden).Total Genomic DNA was extracted by the GUSN-Silica Gel kit.