Serum sex hormone-binding globulin and serum nonsex hormone-binding globulin-bound testosterone fractions in prepubertal boys with chronic renal failure.

We had previously reported that serum sex hormone binding-globulin (SHBG) decreases and serum non-SHBG-bound testosterone (T) and free T increase significantly from infancy to late prepuberty in normal prepubertal children of both sexes. We had also shown an age-related delay in these changes in hypopituitary boys, which was reversed by GH treatment. Stunted growth and delayed puberty are conspicuous features of chronic renal failure (CRF).

Insulin-like growth factor I (IGF-I) and growth in children undergoing hemodialysis or after successful renal transplantation.

To analyze the relationship between IGF-I and growth in children with chronic renal failure, we studied 7 patients undergoing hemodialysis and 7 patients after successful renal transplantation. IGF-I was measured by acid chromatography using ODS silica columns. Hemodialyzed patients grew poorly (mean 1.

Growth in Turner's syndrome: long term treatment with low dose ethinyl estradiol.

The growth response to 100 ng/kg BW X day ethinyl estradiol (2-4 micrograms/day) given for 18 months was studied in a group of nine patients with Turner's syndrome, aged 8.6-13.3 yr.

Somatomedin response to testosterone stimulation in children with male pseudohermaphroditism, cryptorchidism, anorchia, or micropenis.

Somatomedin-C (Sm-C) levels increase during puberty. To determine if testosterone could play a role in the pubertal Sm-C increase, 17 boys with low levels of endogenous testosterone (80 ng/dl) were studied before and 7 days after im testosterone treatment. The serum testosterone levels achieved were comparable to those found during normal puberty in most instances.

Rat liver DNA synthesis during the "catch-up" growth of varied nutritional rehabilitation.

When nutritionally rehabilitated, the malnourished suckling rat shows "catch-up" growth. This acceleration of growth rate was studied at the cellular level after varied nutritional rehabilitation by measuring the incorporation of tritiated thymidine into DNA and the activity of DNA polymerase. Changes in total liver DNA, protein and the protein/DNA ratio were also determined.

Modulatory role for biogenic amines in the cerebral cortex. Microiontophoretic studies.

In order to investigate the mode of action of biogenic amines in rat cerebral cortex, the unitary activity of spontaneously firing neurons and their excitatory response to acetylcholine (ACh) were examined using microiontophoretic administration of dopamine (DA), noradrenaline (NA) and serotonin (5-HT). The predominant effect of these biogenic amines on the spontaneous activity was a profound and prolonged inhibition of firing (2-4 min), which attained its maximum within 15-120 sec. This response was generally more abrupt in onset and of greater magnitude with NA and 5-HT than with DA.

Taurine treatment of spontaneous chronic epilepsy in a cat.

Administration of taurine to a cat suffering from naturally occurring chronic (about 3 years) epilepsy caused disappearance of the clinical manifestation of the seizures as well as a marked improvement in the previously grossly abnormal EEG. The treatment consisted of injections of taurine initially, followed by a more prolonged administration of the amino acid by mouth. The combined methods of administration seemed essential for the success of the trial.

Catecholamines released from cerebral cortex in the cat; decrease during sensory stimulation.

In an attempt to determine the functional role of catecholamine (CA) nerve terminals in cerebral cortex the release of endogenous norepinephrine (NE) and dopamine (DA) into superfusates from visual and somatosensory cortex of the cat have been measured by a sensitive radiometric enzymatic assay based on the methylation of CA by catechol-O-methyltransferase (COMT) in the presence of a [3H]-methyl donor and followed by resolution of 3H derivatives through a series of organic extractions. In the flaxedilized animal maintained under local anaesthesia with artificial respiration the concentration of CA measured in 30-min superfusates was fairly constant in a given experiment under basal conditions without sensory stimulation, but varied widely from one experiment to another. Variations in NE were often independent of those for DA.