Real-Time Monitoring of SARS-CoV-2 Variants in Oklahoma Wastewater through Allele-Specific RT-qPCR.

During the COVID-19 pandemic, wastewater surveillance was used to monitor community transmission of SARS-CoV-2. As new genetic variants emerged, the need for timely identification of these variants in wastewater became an important focus. In response to increased reports of Omicron transmission across the United States, the Oklahoma Wastewater Surveillance team utilized allele-specific RT-qPCR assays to detect and differentiate variants, such as Omicron, from other variants found in wastewater in Oklahoma.

Using Wastewater Surveillance to Monitor Gastrointestinal Pathogen Infections in the State of Oklahoma.

During the COVID-19 pandemic, wastewater surveillance was widely used to monitor temporal and geographical infection trends. Using this as a foundation, a statewide program for routine wastewater monitoring of gastrointestinal pathogens was established in Oklahoma. The results from 18 months of surveillance showed that wastewater concentrations of , and norovirus exhibit similar seasonal patterns to those observed in reported human cases (F = 4-29, < 0.

The effect of Paenibacillus on IDEXX Enterolert results from freshwater stream environments.

Enterolert, a fluorogenic substrate test, is used as a quantitative method for determining freshwater concentrations of Enterococcus for water quality indicators. However, there is some evidence from recent studies suggesting that Enterolert may not suppress false positives due to pollution sources in waterbodies. In this study, we evaluated this method by analyzing field water and sediment samples from four freshwater streams.

Termite sensitivity to temperature affects global wood decay rates.

Deadwood is a large global carbon store with its store size partially determined by biotic decay. Microbial wood decay rates are known to respond to changing temperature and precipitation. Termites are also important decomposers in the tropics but are less well studied.

Predicting COVID-19 cases in diverse population groups using SARS-CoV-2 wastewater monitoring across Oklahoma City.

SARS-CoV-2 was discovered among humans in late 2019 and rapidly spread across the world. Although the virus is transmitted by respiratory droplets, most infected persons also excrete viral particles in their feces. This fact prompted a range of studies assessing the usefulness of wastewater surveillance to determine levels of infection and transmission and produce early warnings of outbreaks in local communities, independently of human testing.

Spatial variability in tree-ring carbon isotope discrimination in response to local drought across the entire loblolly pine natural range.

Considering the temporal responses of carbon isotope discrimination (Δ13C) to local water availability in the spatial analysis of Δ13C is essential for evaluating the contribution of environmental and genetic facets of plant Δ13C. Using tree-ring Δ13C from years with contrasting water availability at 76 locations across the natural range of loblolly pine, we decomposed site-level Δ13C signals to maximum Δ13C in well-watered conditions (Δ13Cmax) and isotopic drought sensitivity (m) as a change in Δ13C per unit change of Palmer's Drought Severity Index (PDSI). Site water status, especially the tree lifetime average PDSI, was the primary factor affecting Δ13Cmax.

Long-term effects of catastrophic wind on southern US coastal forests: Lessons from a major hurricane.

Threats posed by windstorms are an increasing concern to forest managers in the southern United States (US). Studies suggest that the southern US will experience an increase in the occurrence as well as the intensity of windstorms, such as hurricanes, in the future. However, forest managers may have difficulty preparing for this future because there is limited understanding of how windstorms affect the structure and composition of forests over the long term.

Field studies of pollutant removal from nursery and greenhouse runoff by constructed wetlands.

Plant nursery runoff commonly contains pesticides and nutrients that often threaten aquatic ecosystems. Constructed wetlands could be a tool to remove pesticides and nutrients from nursery runoff but have not been extensively studied in this setting. Two field-scale constructed wetlands (one subsurface-flow constructed wetland [SFCW] and one free-surface constructed wetland [FSCW]) were implemented and monitored for water quality improvement.

Urban stormwater characterization, control, and treatment.

This review summarizes over 280 studies published in 2019 related to the characterization, control, and management of urban stormwater runoff. A summary of quantity and quality concerns is provided in the first section of the review, serving as the foundation for the following sections which focus on the control and treatment of stormwater runoff. Finally, the impact of stormwater control devices at the watershed scale is discussed.

Accounting for two-billion tons of stabilized soil carbon.

The pedosphere is the largest terrestrial reservoir of organic carbon, yet soil-carbon variability and its representation in Earth system models is a large source of uncertainty for carbon-cycle science and climate projections. Much of this uncertainty is attributed to local and regional-scale variability, and predicting this variation can be challenging if variable selection is based solely on a priori assumptions due to the scale-dependent nature of environmental determinants. Data mining can optimize predictive modeling by allowing machine-learning algorithms to learn from and discover complex patterns in large datasets that may have otherwise gone unnoticed, thus increasing the potential for knowledge discovery.

Urban stormwater characterization, control, and treatment.

This review summarizes over 250 studies published in 2018 related to the characterization, control, and management of urban stormwater runoff. The review covers three broad themes: (a) quantity and quality characterization of stormwater, (b) control and treatment of stormwater runoff, and (c) implementation and assessment of watershed-scale green stormwater infrastructure (GSI). Each section provides an overview of the 2018 literature, common themes, and future work.

Urban Stormwater Characterization, Control and Treatment.

A review of literature related to stormwater runoff characterization and its subsequent management and treatment from 2017 was conducted. The 250 articles summarized herein are organized along three central themes: (1) stormwater quality and quantity characteristics, (2) site-scale stormwater management practices, with a focus on low impact development (LID) and green infrastructure (GI), and (3) watershed-scale performance of stormwater controls. Within each section, common research themes and future work are highlighted.

Urban Stormwater Characterization, Control, and Treatment.

A summary of 246 studies published in 2016 on topics related to the characterization and management of urban stormwater runoff is presented in the following review. The review is structured along three major topical areas: (1) general characterization of stormwater quantity and quality; (2) engineered systems for stormwater control and treatment, including erosion and sediment control practices, constructed stormwater ponds and wetlands, bioretention, permeable pavement, greenroofs, and rainwater harvesting and (3) watershedscale application of stormwater treatment and control practices. Common research themes and needs highlighted throughout this review include efforts to better understand stormwater transport and treatment mechanisms and their representation in models, advancements to optimize the design of stormwater control measures to meet specific hydrologic and/or water quality targets, and increasing understanding of the biophysical and social factors that influence watershed-scale implementation of low impact development and other stormwater control measures.

Urban Stormwater Characterization, Control, and Treatment.

The following review presents a synthesis of 181 journal articles published during 2015 that represent progress toward better characterizing, controlling, and treating urban stormwater runoff. The review is structured by general topical areas related to (1) stormwater quality and quantity characterization; (2) engineered stormwater control and treatment practices, including erosion and sediment control, stormwater ponds, constructed stormwater wetlands, bioretention, permeable pavement, greenroofs, and rainwater harvesting systems; and (3) watershed-scale modeling and optimization of stormwater control and treatment practices. Common research themes emerging from this collection of studies include potential to enhance hydrologic and pollutant treatment performance of stormwater practices via media amendments and the use of innovative outlet control structures, as well as development of a more mechanistic understanding of hydrologic and water-quality functions to inform modeling and performance predictions.

Association of Soil Aggregation with the Distribution and Quality of Organic Carbon in Soil along an Elevation Gradient on Wuyi Mountain in China.

Forest soils play a critical role in the sequestration of atmospheric CO2 and subsequent attenuation of global warming. The nature and properties of organic matter in soils have an influence on the sequestration of carbon. In this study, soils were collected from representative forestlands, including a subtropical evergreen broad-leaved forest (EBF), a coniferous forest (CF), a subalpine dwarf forest (DF), and alpine meadow (AM) along an elevation gradient on Wuyi Mountain, which is located in a subtropical area of southeastern China.

Critical Review of Technical Questions Facing Low Impact Development and Green Infrastructure: A Perspective from the Great Plains.

Since its inception, Low Impact Development (LID) has become part of urban stormwater management across the United States, marking progress in the gradual transition from centralized to distributed runoff management infrastructure. The ultimate goal of LID is full, cost-effective implementation to maximize watershed-scale ecosystem services and enhance resilience. To reach that goal in the Great Plains, the multi-disciplinary author team presents this critical review based on thirteen technical questions within the context of regional climate and socioeconomics across increasing complexities in scale and function.

Investigation of insecticide leaching from potted nursery stock and aquatic health benefits of bioretention cells receiving nursery runoff.

Tree nurseries and greenhouses within the USDA red imported fire ant (RIFA) quarantine zone are required to incorporate insecticides into their potting media to prevent artificial spread of RIFA. Bifenthrin and fipronil are two common insecticides that are incorporated into potting media. During irrigation and stormwater events, there is potential for insecticides to leach from nursery pots, resulting in the contamination of nearby surface waters.

Molecular detection of Campylobacter spp. and fecal indicator bacteria during the northern migration of sandhill cranes (Grus canadensis) at the central Platte River.

The risk to human health of the annual sandhill crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010.

Supportive breeding boosts natural population abundance with minimal negative impacts on fitness of a wild population of Chinook salmon.

While supportive breeding programmes strive to minimize negative genetic impacts to populations, case studies have found evidence for reduced fitness of artificially produced individuals when they reproduce in the wild. Pedigrees of two complete generations were tracked with molecular markers to investigate differences in reproductive success (RS) of wild and hatchery-reared Chinook salmon spawning in the natural environment to address questions regarding the demographic and genetic impacts of supplementation to a natural population. Results show a demographic boost to the population from supplementation.

Development and evaluation of a quantitative PCR assay targeting sandhill crane (Grus canadensis) fecal pollution.

While the microbial water quality in the Platte River is seasonally impacted by excreta from migrating cranes, there are no methods available to study crane fecal contamination. Here we characterized microbial populations in crane feces using phylogenetic analysis of 16S rRNA gene fecal clone libraries. Using these sequences, a novel crane quantitative PCR (Crane1) assay was developed, and its applicability as a microbial source tracking (MST) assay was evaluated by determining its host specificity and detection ability in environmental waters.

The effect of permafrost thaw on old carbon release and net carbon exchange from tundra.

Permafrost soils in boreal and Arctic ecosystems store almost twice as much carbon as is currently present in the atmosphere. Permafrost thaw and the microbial decomposition of previously frozen organic carbon is considered one of the most likely positive climate feedbacks from terrestrial ecosystems to the atmosphere in a warmer world. The rate of carbon release from permafrost soils is highly uncertain, but it is crucial for predicting the strength and timing of this carbon-cycle feedback effect, and thus how important permafrost thaw will be for climate change this century and beyond.