Publications by authors named "Jason Lequyer"
Article Synopsis
- Image noise is a significant issue in light microscopy, especially for live-cell imaging, requiring low-light conditions for cell viability.
- Traditional denoisers rely on large training datasets, which are hard to gather in real-time imaging scenarios.
- Noise2Fast introduces a new approach using 'chequerboard downsampling' to train on fewer images, enhancing speed while maintaining accuracy, making it suitable for real-time multi-modal imaging applications.
High-throughput functional characterization of genetic variants in their endogenous locus has so far been possible only with methods that rely on homology-directed repair, which are limited by low editing efficiencies. Here, we adapted CRISPR prime editing for high-throughput variant classification and combined it with a strategy that allows for haploidization of any locus, which simplifies variant interpretation. We demonstrate the utility of saturation prime editing (SPE) by applying it to the NPC intracellular cholesterol transporter 1 gene (NPC1), mutations in which cause the lysosomal storage disorder Niemann-Pick disease type C.
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