Identification of Antimicrobial Resistance-Associated Genes through Whole Genome Sequencing of Isolates with Different Antimicrobial Resistances.

Antimicrobial resistance (AMR) in has been previously associated with topoisomerase and ribosomal gene mutations rather than specific resistance-conferring genes. Using whole genome sequencing (WGS) to identify potential new AMR mechanisms for it was found that a 2019 clinical isolate with high MIC (2019-043682) for fluoroquinolones, macrolides, lincosamides, pleuromutilins and tetracyclines had a new core genome multilocus sequencing (cgMLST) type (ST10-like) and 91% sequence similarity to the published genome of PG45. Closely related to PG45, a 1982 isolate (1982-M6152) shared the same cgMLST type (ST17), 97.

Diversity of strains from pigs across Ontario, Canada.

causes highly contagious swine enzootic pneumonia worldwide. It has been reported that highly diversified strains exist in different parts of the world. We found gene sequencing analysis, an affordable and simple-to-perform typing method, to be specific and highly sensitive when applied to the molecular typing of 113 -positive clinical samples directly without culture.