Thermodynamics and kinetics of adaptive binding in the malachite green RNA aptamer.

Adaptive binding, the ability of molecules to fold themselves around the structure of a ligand and thereby incorporating it into their three-dimensional fold, is a key feature of most RNA aptamers. The malachite green aptamer (MGA) has been shown to bind several closely related triphenyl dyes with planar and nonplanar structures in this manner. Competitive binding studies using isothermal titration calorimetry and stopped flow kinetics have been conducted with the aim of understanding the adaptive nature of RNA-ligand interaction.

A two-year follow-up study and prospective evaluation of the DSM-IV axis V.

A six-month cohort of general adult psychiatric inpatients was followed for up to two years to evaluate outcome and contrast the validity of DSM-IV measures of adaptive functioning-the Global Assessment of Functioning (GAF), the Social and Occupational Functioning Assessment Scale (SOFAS), and the Global Assessment of Relational Functioning Scale (GARF). Detailed data, including quality-of-life ratings and DSM-IV axis I and V codes, were collected by interview and self-report questionnaires for 53 study participants. Patients' retrospective ratings of the care they received were not predictive of outcome.