A novel ex-vivo porcine renal xenotransplantation model using a pulsatile machine preservation system.

Background: Animal models to investigate pathophysiology and xenotransplantation require complex techniques and significant animal utilization. The aim of the study was to develop a reliable ex-vivo technique to test xenotransplant interventions.

Material/methods: Miniature Swine being utilized for a nonsurvival study acted as donor animals.

Hypothermic machine preservation attenuates ischemia/reperfusion markers after liver transplantation: preliminary results.

Background: Hypothermic machine perfusion (HMP) has shown significant benefits in renal transplantation but is still in its infancy in liver transplantation. Potential benefits include diminished preservation injury and improved early graft function.

Methods: We analyzed liver tissue and effluent collected during our Phase 1 trial of liver HMP.