Publications by authors named "Jasmine K Ahluwalia"
We had previously proposed that the post-transcriptional regulation through microRNA as a mechanism for incomplete penetrance and variable expressivity, leads to lack of correlation between genotype and phenotype. Here we report the validation of miRNA-target interactions we predicted earlier and demonstrate the regulation of endogenous JAG1 by hsa-miR-214 and hsa-miR-124, and TGFBR2 by hsa-miR-34b*, through luciferase activity of reporter constructs and also the expression levels of the endogenous genes. Using these targets, we have modeled the diploid state for miRNA target site with heterozygosity for the SNP and demonstrate the differential targeting of an otherwise identical 3'UTR.
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- Incomplete penetrance and variable expressivity are genetic concepts that explain why individuals with the same genotype can have different traits or symptoms, especially in dominant diseases.
- The differences in gene expression can be influenced by factors like microRNA, which help regulate how genes are turned on or off at the translational level.
- The study suggests that the presence of specific miRNA binding sites and single nucleotide polymorphisms in these sites may affect how genes with the same genetic code are expressed, leading to observable variations in phenotype.
Background: Cellular miRNAs play an important role in the regulation of gene expression in eukaryotes. Recently, miRNAs have also been shown to be able to target and inhibit viral gene expression. Computational predictions revealed earlier that the HIV-1 genome includes regions that may be potentially targeted by human miRNAs.
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