Simultaneous Production of a Virus-Like Particle Linked to dsRNA to Enhance dsRNA Delivery for Yellow Head Virus Inhibition.

A co-expressed densovirus (DNV) capsid and dsRNA specific to the yellow head virus (YHV) (CoEx cpDNV/ds) has been shown to suppress YHV replication in the Pacific white-legged shrimp (). However, maintaining two plasmids in a single bacterial cell is not desirable; therefore, a single plasmid harboring both the DNV capsid and the dsRNA-YHV- gene was constructed under the regulation of a single T7 promoter, designated pET28a-Linked cpDNV-ds. Following induction, this novel construct expressed an approximately 37-kDa recombinant protein associated with a roughly 400-bp dsRNA (Linked cpDNV-ds).